Document Detail


The seed coat of Phaseolus vulgaris interferes with the development of the cowpea weevil [Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)].
MedLine Citation:
PMID:  15048195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have confirmed here that the seeds of the common bean (Phaseolus vulgaris, L.) do not support development of the bruchid Callosobruchus maculatus (F.), a pest of cowpea [Vigna unguiculata (L.) Walp] seeds. Analysis of the testa (seed coat) of the bean suggested that neither thickness nor the levels of compounds such as tannic acid, tannins, or HCN are important for the resistance. On the other hand, we have found that phaseolin (vicilin-like 7S storage globulin), detected in the testa by Western blotting and N-terminal amino acid sequencing, is detrimental to the development of C. maculatus. As for the case of other previously studied legume seeds (Canavalia ensiformis and Phaseolus lunatus) we suggest that the presence of vicilin-like proteins in the testa of P. vulgaris may have had a significant role in the evolutionary adaptation of bruchids to the seeds of leguminous plants.
Authors:
Luciana B Silva; Maurício P Sales; Antônia E A Oliveira; Olga L T Machado; Kátia V S Fernandes; José Xavier-Filho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-03-04
Journal Detail:
Title:  Anais da Academia Brasileira de Ciências     Volume:  76     ISSN:  0001-3765     ISO Abbreviation:  An. Acad. Bras. Cienc.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-29     Completed Date:  2004-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7503280     Medline TA:  An Acad Bras Cienc     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  57-65     Citation Subset:  IM    
Affiliation:
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28015-620, Campos dos Goytacazes, RJ, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Beetles / drug effects*
Blotting, Western
Crops, Agricultural / parasitology*
Electrophoresis, Polyacrylamide Gel
Female
Phaseolus / chemistry*,  parasitology
Plant Proteins / isolation & purification,  pharmacology*
Seed Storage Proteins
Seeds / chemistry,  parasitology
Chemical
Reg. No./Substance:
0/Plant Proteins; 0/Seed Storage Proteins; 104042-75-3/phaseolin protein, Phaseolus vulgaris; 9067-60-1/vicilin protein, plant

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