| A secreted luciferase for ex vivo monitoring of in vivo processes. | |
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MedLine Citation:
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PMID: 18204457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Luciferases are widely used to monitor biological processes. Here we describe the naturally secreted Gaussia princeps luciferase (Gluc) as a highly sensitive reporter for quantitative assessment of cells in vivo by measuring its concentration in blood. The Gluc blood assay complements in vivo bioluminescence imaging, which has the ability to localize the signal and provides a multifaceted assessment of cell viability, proliferation and location in experimental disease and therapy models. |
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Authors:
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Thomas Wurdinger; Christian Badr; Lisa Pike; Ruben de Kleine; Ralph Weissleder; Xandra O Breakefield; Bakhos A Tannous |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-01-20 |
Journal Detail:
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Title: Nature methods Volume: 5 ISSN: 1548-7105 ISO Abbreviation: Nat. Methods Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-31 Completed Date: 2008-02-21 Revised Date: 2011-01-25 |
Medline Journal Info:
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Nlm Unique ID: 101215604 Medline TA: Nat Methods Country: United States |
Other Details:
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Languages: eng Pagination: 171-3 Citation Subset: IM |
Affiliation:
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Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St., Charlestown, Massachusetts 02129, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Angiosperms
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enzymology*,
genetics Animals Cell Line, Tumor Fluorescent Dyes Genes, Reporter* Glioma / metabolism*, pathology Humans Luciferases, Bacterial / genetics, metabolism* Luminescent Measurements / methods* Mice Mice, Nude Microscopy, Fluorescence / methods* |
| Grant Support | |
ID/Acronym/Agency:
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1K99CA126839-01/CA/NCI NIH HHS; P50 CA086355-02S1/CA/NCI NIH HHS; P50 CA86355-04/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Luciferases, Bacterial |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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