| The secreted Helicobacter cysteine-rich protein A causes adherence of human monocytes and differentiation into a macrophage-like phenotype. | |
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MedLine Citation:
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PMID: 19393649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Helicobacter pylori genomes typically contain 8 or 9 genes that code for secreted and highly disulfide-bridged proteins designated Helicobacter cysteine-rich proteins (Hcp). Here we show that HcpA (hp0211) but not HcpC (hp1098) triggers the differentiation of human myeloid Thp1 monocytes into macrophages. Small amounts of HcpA cause the transition of round-shaped monocytes into cells with star-like morphologies, adherence to the culture dish surface, phagocytosis of opsonized fluorescent microspheres, and expression of the surface marker protein CD11b, all of which are indicative of a macrophage-like phenotype. We conclude that HcpA acts as a bacterial immune modulator similar to a eukaryotic cytokine. |
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Authors:
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Claudia Dumrese; Lutz Slomianka; Urs Ziegler; Sung Sook Choi; Awdhesh Kalia; Alma Fulurija; Wei Lu; Douglas E Berg; Mohammed Benghezal; Barry Marshall; Peer R E Mittl |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-04-24 |
Journal Detail:
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Title: FEBS letters Volume: 583 ISSN: 1873-3468 ISO Abbreviation: FEBS Lett. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-18 Completed Date: 2009-06-18 Revised Date: 2011-01-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1637-43 Citation Subset: IM |
Affiliation:
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Center for Microscopy and Image Analysis, University of Zürich, Winterthurer Strasse 190, Zürich, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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metabolism* Cell Adhesion Cell Differentiation* Helicobacter pylori / metabolism* Humans Macrophages / cytology*, metabolism Microscopy, Electron, Scanning Monocytes / cytology*, metabolism Phagocytosis Phenotype Recombinant Proteins / genetics, metabolism beta-Lactamases / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK 63041/DK/NIDDK NIH HHS; R01 DK063041-01/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Recombinant Proteins; EC 3.5.2.-/hcpA protein, Helicobacter pylori; EC 3.5.2.6/beta-Lactamases |
| Comments/Corrections | |
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