| The second cysteine protease inhibitor, EhICP2, has a different localization in trophozoites of Entamoeba histolytica than EhICP1. | |
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MedLine Citation:
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PMID: 16802137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The genome of Entamoeba histolytica contains two genes encoding inhibitors of cysteine proteases of the chagasin family. In contrast to that of EhICP1, the derived primary structure of the second inhibitor, EhICP2, possesses a typical N-terminal signal sequence. Processed EhICP2 is as weakly related to amoebiasin-1 (27% identity) as to chagasin (identity 30%), indicating a different evolutionary origin of both amebic genes. By Northern blots, we confirmed the expression of the ehicp2 gene, and in Western blots, the presence of the 11.5-kDa protein in trophozoite extracts was demonstrated. The inhibitor localized to large intracellular structures clearly differs from those containing EhICP1 as shown by indirect immunofluorescence. Recombinant EhICP2 significantly inhibited the cysteine protease activity of the amebic cell extract but with a lower extent than EhICP1. An overlay assay using a crude trophozoite extract demonstrated binding affinity of the amebic cysteine protease EhCP1 to EhICP2. |
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Authors:
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M Sarić; A Vahrmann; I Bruchhaus; T Bakker-Grunwald; H Scholze |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-27 |
Journal Detail:
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Title: Parasitology research Volume: 100 ISSN: 0932-0113 ISO Abbreviation: Parasitol. Res. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-19 Completed Date: 2007-11-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 171-4 Citation Subset: IM |
Affiliation:
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Department of Biology/Chemistry, University of Osnabrueck, 49069, Osnabrueck, Germany, scholze@biologie.uni-osnabrueck.de. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AJ634054; AJ890473 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cysteine Proteinase Inhibitors / chemistry, genetics*, isolation & purification Entamoeba histolytica / growth & development, physiology* Kinetics Molecular Sequence Data Protozoan Proteins / chemistry, genetics*, isolation & purification Sequence Alignment Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/Cysteine Proteinase Inhibitors; 0/Protozoan Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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