| The second chromophore in Drosophila photolyase/cryptochrome family photoreceptors. | |
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MedLine Citation:
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PMID: 22175817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The photolyase/cryptochrome family of proteins are FAD-containing flavoproteins which carry out blue-light-dependent functions including DNA repair, plant growth and development, and regulation of the circadian clock. In addition to FAD, many members of the family contain a second chromophore which functions as a photo-antenna, harvesting light and transferring the excitation energy to FAD and thus increasing the efficiency of the system. The second chromophore is methenyltetrahydrofolate (MTHF) in most photolyases characterized to date and FAD, FMN, or 5-deazariboflavin in others. To date, no second chromophore has been identified in cryptochromes. Drosophila contains three members of the cryptochrome/photolyase family: cyclobutane pyrimidine dimer (CPD) photolyase, (6-4) photoproduct photolyase, and cryptochrome. We developed an expression system capable of incorporating all known second chromophores into the cognate cryptochrome/photolyase family members. Using this system, we demonstrate that Drosophila CPD photolyase and (6-4) photolyase employ 5-deazariboflavin as their second chromophore, but Drosophila cryptochrome, which is evolutionarily closer to (6-4) photolyase than the CPD photolyase, lacks a second chromophore. |
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Authors:
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Christopher P Selby; Aziz Sancar |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-12-27 |
Journal Detail:
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Title: Biochemistry Volume: 51 ISSN: 1520-4995 ISO Abbreviation: Biochemistry Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-01-10 Completed Date: 2012-03-08 Revised Date: 2012-03-13 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 167-71 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599-7260, United States. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arabidopsis Proteins / chemistry Baculoviridae Catalysis Cryptochromes / chemistry* Deoxyribodipyrimidine Photo-Lyase / chemistry* Drosophila Proteins / chemistry*, genetics Drosophila melanogaster / enzymology, genetics Photoreceptor Cells, Invertebrate / enzymology*, virology Proton-Translocating ATPases / chemistry Pyrimidine Dimers / chemistry Steroid Hydroxylases / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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GM31082/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/CRY2 protein, Arabidopsis; 0/Cryptochromes; 0/Drosophila Proteins; 0/Pyrimidine Dimers; EC 1.14.-/CPD protein, Arabidopsis; EC 1.14.-/Steroid Hydroxylases; EC 3.6.3.14/Proton-Translocating ATPases; EC 4.1.99.3/Deoxyribodipyrimidine Photo-Lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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