Document Detail


A screening for the occurrence of autoreactive antibodies in sera of pregnant and non-pregnant bitches.
MedLine Citation:
PMID:  16420328     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sera of healthy pregnant (group I, n = 11) and non-pregnant (group II, n = 11) bitches were screened for autoantibodies (AAb). In both groups, blood samples were drawn every fifth day between days 5 and 55 after mating. Serum was analysed via indirect immunofluorescence (IIF) with the Canine ANA HEp-2 Screening Kit. In all animals, anticytoplasmic AAb were detected. Utilizing primate-heart substrate slides AAb against contractile proteins of the cytoplasm could be observed. The predominating fluorescence pattern in pregnant animals resembled above all desmin, which was proven via Western blot. The sera were then pre-incubated with tropomyosin, actin, vimentin, vinculin and keratin solutions, and assessed on HEp-2 slides and on human and canine fibroblasts as well. The latter substrate was used to verify whether the detected Ab were in fact AAb. Utilizing tropomyosin, revealed elimination of the cytoplasmic fluorescences on all three substrates. It is therefore assumed, that in sera of healthy dogs, AAb against contractile structure proteins of the cytoplasm are present regularly. The majority of pregnant bitches presented with higher end-point titres (EPT), than to be found in non-pregnant dogs. AAb against desmin played the key role in those patterns. In addition, sera were screened for thyroid specific AAb, namely thyroglobulin, thyroid peroxidase (TPO), T3 and T4, and for AAb against insulin by ELISA or Western blot (TPO). Only in two of the pregnant bitches a weak positive reaction (1:100) for T3-AAb was detected.
Authors:
S Schäfer-Somi; K R Herkner; S Neubauer; M Egerbacher; M Patzl; S Pollak; O Ali Aksoy; H B Beceriklisoy; H Kanca; M Findik; H Kalender; S Aslan
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  41     ISSN:  0936-6768     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-19     Completed Date:  2006-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  48-54     Citation Subset:  IM    
Affiliation:
Clinic for Obstetrics, Gynecology and Andrology, University for Veterinary Medicine, Vienna, Austria. sabine.schaefer@vu-wien.ac.at
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoantibodies / blood*,  immunology*
Dogs / blood*,  immunology
Electrophoresis, Polyacrylamide Gel / methods,  veterinary
Enzyme-Linked Immunosorbent Assay / methods,  veterinary
Female
Fluorescent Antibody Technique, Indirect / methods,  veterinary
Immunologic Factors / blood*,  immunology
Molecular Weight
Pregnancy
Pregnancy, Animal / blood*,  immunology
Seroepidemiologic Studies
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Immunologic Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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