Document Detail


A screening method for biotinidase deficiency in newborns.
MedLine Citation:
PMID:  6690118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a method for neonatal screening for biotinidase (EC 3.5.1.12) deficiency. Biotinidase activity is assessed colorimetrically from dried samples of whole blood spotted on the same filter papers as used in the neonatal screening for phenylketonuria. After the reaction, samples from normal infants are characteristically purple, whereas those from affected individuals are straw-colored. To confirm the deficiency, the enzyme is quantitatively assayed in additional blood spots or serum. A pilot study has been initiated with samples obtained by the Commonwealth of Virginia for phenylketonuria testing.
Authors:
G S Heard; J R Secor McVoy; B Wolf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  30     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-02-15     Completed Date:  1984-02-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  125-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / blood,  deficiency*
Biotinidase
Clinical Enzyme Tests*
Colorimetry
Humans
Infant, Newborn
Mass Screening / economics,  methods*
Metabolism, Inborn Errors / diagnosis*
Pilot Projects
Virginia
Grant Support
ID/Acronym/Agency:
AM 00677/AM/NIADDK NIH HHS; AM 25675/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
EC 3.5.-/Amidohydrolases; EC 3.5.1.12/Biotinidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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