Document Detail


A screening approach reveals the influence of mineral coating morphology on human mesenchymal stem cell differentiation.
MedLine Citation:
PMID:  23420758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
"Biomimetic" inorganic coating on biomaterials has been an active area of research with the aim of providing bioactive surfaces that can regulate cell behavior. Previous studies have demonstrated that human mesenchymal stem cell (hMSC) behavior is differentially regulated by the physical and chemical properties of inorganic mineral coatings, indicating that modulation of mineral properties is potentially important in regulating hMSC behavior. However, the lack of an efficient experimental context, in which to study stem cell behavior on inorganic substrates, has made it difficult to systematically study the effects of specific mineral coating parameters on hMSC behavior. In this study, we developed an efficient experimental platform to screen for the effects of mineral coating morphology on hMSC expansion and differentiation. hMSC expansion on mineral coatings was regulated by the micro-scale morphology of these coatings, with greater expansion on small granule-like coatings when compared to plate-like or net-like coatings. In contrast, hMSC osteogenic differentiation was inversely correlated with cell expansion on mineral coatings indicating that mineral coating morphology was a key parameter that regulates hMSC differentiation. The effect of mineral coating morphology on hMSC behavior underlines the utility of this inorganic screening platform to identify optimal coatings for medical devices and bone tissue engineering applications.
Authors:
Siyoung Choi; William L Murphy
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-07
Journal Detail:
Title:  Biotechnology journal     Volume:  8     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-04     Completed Date:  2013-09-16     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  496-501     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Body Fluids
Calcium Phosphates / pharmacology
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Coated Materials, Biocompatible / pharmacology*
DNA / analysis
Humans
Materials Testing / methods
Mesenchymal Stromal Cells / drug effects*,  physiology*
Minerals / pharmacology*
Models, Biological
Surface Properties
Grant Support
ID/Acronym/Agency:
R01 AR059916/AR/NIAMS NIH HHS; R01AR059916/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Phosphates; 0/Coated Materials, Biocompatible; 0/Minerals; 9007-49-2/DNA; 97Z1WI3NDX/calcium phosphate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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