| The scavenger receptors SRA-1 and SREC-I cooperate with TLR2 in the recognition of the hepatitis C virus non-structural protein 3 by dendritic cells. | |
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MedLine Citation:
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PMID: 20338659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUNDS & AIMS: The hepatitis C virus NS3 protein is taken up by myeloid cells in a TLR2-independent manner and activates myeloid cells via TLR2. This study aimed to identify the endocytic receptor(s) involved in the uptake of NS3 by myeloid cells and its relation with TLR2. METHODS: Inhibitors and transfected cells were used to identify the nature of the NS3-binding receptors expressed by myeloid cells. The cooperation between scavenger receptors (SRs) and TLR2 in the NS3-mediated activation of myeloid cells was evaluated using inhibitors, cells from TLR2(-/-) mice, and confocal microscopy. The involvement of SRs in NS3 cross-presentation was evaluated in vitro using an NS3-specific human T-cell clone. RESULTS: We observed that SRs are the main binding structures for NS3 on myeloid cells and identified the SRs SRA-1 and SREC-I as endocytic receptors for NS3. Moreover, both SRs and TLR2 cooperate in NS3-induced myeloid cell activation. CONCLUSION: This study highlights a central role for SRs in NS3 uptake and cross-presentation, and demonstrates a tightly orchestrated cooperation between signalling and endocytic innate receptors in NS3 recognition. |
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Authors:
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Céline Beauvillain; Francesca Meloni; Jean-Claude Sirard; Simon Blanchard; Ulrich Jarry; Mari Scotet; Giovanni Magistrelli; Yves Delneste; Vincenzo Barnaba; Pascale Jeannin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-04 |
Journal Detail:
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Title: Journal of hepatology Volume: 52 ISSN: 1600-0641 ISO Abbreviation: J. Hepatol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-03 Completed Date: 2010-08-10 Revised Date: 2012-08-24 |
Medline Journal Info:
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Nlm Unique ID: 8503886 Medline TA: J Hepatol Country: England |
Other Details:
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Languages: eng Pagination: 644-51 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Institut National de la Santé et de la Recherche Médicale, Unité 892, Centre de Recherche en Cancérologie Nantes-Angers, Bâtiment Montéclair, 4 rue Larrey, Angers 49933, France. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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physiology* Animals Antigens, CD14 / immunology Bone Marrow Cells / cytology, immunology CHO Cells Cell Differentiation Cricetinae Cricetulus Dendritic Cells / cytology, immunology*, virology Endocytosis Hepacivirus / immunology* Humans Mice Monocytes / cytology, physiology Myeloid Cells / physiology Receptors, Scavenger / immunology*, metabolism Recombinant Proteins / immunology Scavenger Receptors, Class F / physiology* Toll-Like Receptor 2 / physiology* Transfection Viral Nonstructural Proteins / genetics, immunology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Antigens, CD14; 0/CYFIP1 protein, human; 0/NS3 protein, hepatitis C virus; 0/Receptors, Scavenger; 0/Recombinant Proteins; 0/SCARF1 protein, human; 0/Scavenger Receptors, Class F; 0/TLR2 protein, human; 0/Toll-Like Receptor 2; 0/Viral Nonstructural Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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