Document Detail

The scavenger receptor, cysteine-rich domain-containing molecule gp-340 is differentially regulated in epithelial cell lines by phorbol ester.
MedLine Citation:
PMID:  12452835     Owner:  NLM     Status:  MEDLINE    
Gp-340 is a glycoprotein belonging to the scavenger receptor cysteine rich (SRCR) group B family. It binds to host immune components such as lung surfactant protein D (SP-D). Recent studies found that gp-340 interacts directly with pathogenic microorganisms and induces their aggregation, suggesting its involvement in innate immunity. In order to investigate further its potential immune functions in the appropriate cell lines, the expression of gp-340 in four conventional immune cell lines (U937, HL60, Jurkat, Raji), and two innate immune-related epithelial cell lines (A549 derived from lung and AGS from stomach), was examined by RT-PCR and immunohistochemistry. The resting immune cell lines showed weak or no gp-340 mRNA expression; while the two epithelial cell lines expressed gp-340 at much higher level, which was differentially regulated by phorbol myristate acetate (PMA) treatment. In the A549 cells, gp-340 was up-regulated along with the PMA-induced proinflammatory expression of both IL-6 and IL-8. In AGS cells, PMA down-regulation of gp-340 was seen in parallel with an up-regulation of the two mature gastric epithelial specific proteins TFF1 (trefoil factor 1) and TFF2, which are implicated as markers of terminal differentiation. Analysis of the distribution of gp-340, together with the TFFs and SP-D in normal lung and gastric mucosa, supported further our in vitro data. We conclude that the differential regulation of gp-340 in the two epithelial cell lines by PMA indicates that gp-340 s involvement in mucosal defence and growth of epithelial cells may vary at different body locations and during different stages of epithelial differentiation.
W Kang; O Nielsen; C Fenger; J Madsen; S Hansen; I Tornoe; P Eggleton; K B M Reid; U Holmskov
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  130     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-27     Completed Date:  2003-03-03     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  449-58     Citation Subset:  IM    
MRC Immunochemistry Unit, Department of Biochemistry, Oxford University, Oxford, UK.
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MeSH Terms
B-Lymphocytes / chemistry
Biological Markers / analysis
Carcinogens / pharmacology
Cell Division
Cell Line
Dose-Response Relationship, Drug
Epithelial Cells / chemistry*,  cytology,  drug effects
Gastric Mucosa / chemistry
Gene Expression Regulation / drug effects
Growth Substances / analysis,  genetics
Immunohistochemistry / methods
Interleukin-6 / analysis
Interleukin-8 / analysis
Jurkat Cells
Lymphocyte Activation*
Lymphocytes / chemistry*
Microscopy, Phase-Contrast
Muscle Proteins*
Peptides / analysis,  genetics
RNA, Messenger / analysis
Receptors, Immunologic / analysis*,  genetics
Respiratory Mucosa / chemistry
Reverse Transcriptase Polymerase Chain Reaction
Stimulation, Chemical
T-Lymphocytes / chemistry
Tetradecanoylphorbol Acetate / pharmacology
Tumor Suppressor Proteins
Reg. No./Substance:
0/Biological Markers; 0/Carcinogens; 0/Growth Substances; 0/Interleukin-6; 0/Interleukin-8; 0/Mucins; 0/Muscle Proteins; 0/Neuropeptides; 0/Peptides; 0/Proteins; 0/RNA, Messenger; 0/Receptors, Immunologic; 0/TFF1 protein, human; 0/TFF3 protein, rat; 0/Tumor Suppressor Proteins; 0/glycoprotein 340; 146046-78-8/trefoil factor; 16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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