Document Detail

A scanning electron microscope study of normal and SV40-infected human keratinocytes.
MedLine Citation:
PMID:  6195729     Owner:  NLM     Status:  MEDLINE    
We have studied the changes in the surface ultrastructure of human epidermal keratinocytes after infection by the oncogenic virus, SV40. The surfaces of uninfected cells show patterns of dense microvilli and microridges in varying proportion with microridges predominating in the center of the developing keratinocyte colony and on the fully mature keratinocytes (squames) and the microvilli being more prevalent on the immature proliferative cells at the colony periphery. As the transformation process progressed over time highly microridged surfaces became less numerous. After many months in culture cell surfaces were found to exhibit more sparse, predominantly villous ultrastructure. The surface characteristics of the infected cells in long term culture were similar to those observed in a cell line derived from a squamous cell carcinoma.
M L Steinberg; N Cassai; V Defendi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Scanning electron microscopy     Volume:  -     ISSN:  0586-5581     ISO Abbreviation:  Scan Electron Microsc     Publication Date:  1983  
Date Detail:
Created Date:  1983-12-20     Completed Date:  1983-12-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0371617     Medline TA:  Scan Electron Microsc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  343-9     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Squamous Cell / ultrastructure*
Cell Membrane / ultrastructure
Cell Transformation, Neoplastic*
Cells, Cultured
Infant, Newborn
Keratins / physiology*
Microscopy, Electron, Scanning
Microvilli / ultrastructure
Simian virus 40 / genetics*
Skin / microbiology,  ultrastructure*
Grant Support
2-P01-CA 16239/CA/NCI NIH HHS; CA 27869-01/CA/NCI NIH HHS; J-T32-CA 09161/CA/NCI NIH HHS
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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