Document Detail


The scaffold protein c-Jun NH2-terminal kinase-associated leucine zipper protein regulates cell migration through interaction with the G protein G(alpha 13).
MedLine Citation:
PMID:  18826971     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Scaffold proteins for MAP kinase (MAPK) signalling modules play an important role in the specific and efficient signal transduction of the relevant MAPK cascades. Here, we investigated the function of the scaffolding protein c-Jun NH(2)-terminal kinase (JNK)-associated leucine zipper protein (JLP) by depleting it in cultured cells using a short hairpin RNA (shRNA) against human JLP. HeLa and DLD-1 cells stably expressing the shRNA showed a defect in cell migration. The re-expression of full-length shRNA-resistant mouse JLP rescued the impaired cell migration of the JLP-depleted HeLa cells; whereas, a C-terminal deletion mutant of mouse JLP, which failed to bind the G protein G(alpha13), showed little or no effect on the cell migration defect. Furthermore, although a constitutively active G(alpha13) enhanced the migration of control HeLa cells, the G(alpha13)-induced cell migration was significantly suppressed in the JLP-depleted HeLa cells. Taken together, these results suggest that JLP regulates cell migration through an interaction with G(alpha13).
Authors:
Davaakhuu Gantulga; Baljinnyam Tuvshintugs; Yoshio Endo; Takahisa Takino; Hiroshi Sato; Seishi Murakami; Katsuji Yoshioka
Publication Detail:
Type:  Journal Article     Date:  2008-09-30
Journal Detail:
Title:  Journal of biochemistry     Volume:  144     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-10     Completed Date:  2009-04-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  693-700     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Biology, Cancer Research Institute, Kanazawa University, Kanazawa, 920-0934, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / genetics,  metabolism*
Animals
Cell Line, Tumor
Cell Movement
Cells, Cultured
Fluorescent Antibody Technique
GTP-Binding Protein alpha Subunits, G12-G13 / metabolism*
Hela Cells
Humans
Mice
Signal Transduction
rac1 GTP-Binding Protein / metabolism
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/SPAG9 protein, human; 0/Spag9 protein, mouse; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, G12-G13; EC 3.6.5.2/rac1 GTP-Binding Protein

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