| The same luteinized granulosa-luteal cells from normal and superovulated human chorionic gonadotropin (hCG)-stimulated cycles contain hCG and insulin-like growth factor binding protein-1. | |
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MedLine Citation:
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PMID: 10231054 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine whether hCG and IGFBP-1 appear in the same or different cells and in what sequence. DESIGN: Retrospective analysis of laboratory data. SETTING: University medical center. PATIENT(S): Twenty-five women undergoing IVF-ET with natural cycles and 25 women having stimulated IVF-ET. INTERVENTION(S): Cells were obtained from dominant follicles in women with natural cycles and from the follicles from hMG- and hCG-stimulated cycles. MAIN OUTCOME MEASURE(S): Detection and localization of hCG and IGFBP-1 in granulosa-luteal cells using double immunocytochemical staining. Measurement of hCG and IGFBP-1 in follicular fluid and serum. RESULT(S): Three types of hCG staining were found: on the cell surface, on the cell surface and in the cytoplasm, and in the cytoplasm alone. IGFBP-1 stained diffusely in the cytoplasm and was found only in those cells that were luteinized and contained hCG. IGFBP-1 and hCG were colocalized in the same cells. There was a positive correlation between follicular fluid hCG and IGFBP-1 levels, but only in natural IVF-ET cycles. CONCLUSION(S): HCG-driven luteinization is required for IGFBP-1 synthesis to take place in granulosa cells. |
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Authors:
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K Gersak; M Seppälä; M Us-Krasovec |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Fertility and sterility Volume: 71 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-05-12 Completed Date: 1999-05-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 902-6 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University Medical Center, Ljubljana, Slovenia. ksenija.gersak@s-net.net |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Chorionic Gonadotropin / blood, metabolism*, therapeutic use Female Granulosa Cells / metabolism* Humans Immunohistochemistry Insulin-Like Growth Factor Binding Protein 1 / blood, metabolism* Luteal Cells / metabolism* Ovulation Induction / methods* Retrospective Studies |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/Insulin-Like Growth Factor Binding Protein 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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