| The salt-stress induced lysophosphatidic-acid response in Chlamydomonas is produced via phospholipase-A2 hydrolysis of diacylglycerol kinase-generated phosphatidic acid. | |
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MedLine Citation:
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PMID: 21900174 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The unicellular green alga Chlamydomonas has frequently been used as a eukaryotic model system to study intracellular phospholipid signaling pathways in response to environmental stresses. Earlier, we found that hypersalinity induced a rapid increase in the putative lipid second messenger, phosphatidic acid (PA), which was suggested to be generated via activation of a phospholipase D (PLD) pathway and the combined action of a phospholipase C/diacylglycerol kinase (PLC/DGK) pathway. Lysophos-phatidic acid (LPA) was also increased, and was suggested to reflect a phospholipase A2 (PLA2) activity on the basis of pharmacological evidence. The question of PA and LPAs origin is, however, more complicated, especially since both function as precursor in the biosynthesis of phospho- and galactolipids. To address this complexity, a combination of in vivo 32P-radiolabeling and fatty acid-molecular species analyses were performed. Evidence is subsequently provided that LPA is formed from a distinct pool of PA, characterized by a high α-linolenic acid (18:3n-3) content. This molecular species was also highly enriched in the polyphosphoinositide fraction, which is the substrate for PLC to form DAG. Differential 32P-radiolabeling studies and PLD-transphosphatidylation assays were consitent with the hypothesis that the salt-induced LPA response is primarily generated through PLA2-mediated hydrolysis of a DGK-generated PA, and argued against major contributions from PLD or de novo synthesis via ER- or plastid-localized routes. |
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Authors:
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Steven A Arisz; Teun Munnik |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-6 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Swammerdam Institute of Life Sciences, University of Amsterdam, Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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