| A safer methylation procedure with boron trifluoride-methanol reagent for gas chromatographic analysis of ritalinic acid in urine. | |
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MedLine Citation:
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PMID: 1391098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ritalinic acid (RITA), the major metabolite of methylphenidate (Ritaline) is extracted with a solid-phase C8 column. Following elution, methylation of the carboxylic group is performed with boron trifluoride-methanol as a reagent. The methyl ester of RITA which structurally corresponds to the parent compound methylphenidate is reextracted at pH 9-11. Each step of the analytical method has been systematically studied, particularly the parameters governing the esterification reaction with BF3-methanol. The recovery from the overall method is 96%. The limit of detection is 0.05 micrograms ml-1 with GC-NPD for a 10-ml urine sample. The method has been successfully applied to the detection and quantitation of RITA in urine specimens. The specificity of the methylated RITA peak has been verified by GC-MS in scan mode. Use of diazomethane or sulphuric acid is thus replaced by the use of a less hazardous reagent. |
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Authors:
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T Vu-Duc; A Vernay |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 10 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1992 Feb-Mar |
Date Detail:
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Created Date: 1992-10-29 Completed Date: 1992-10-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 187-91 Citation Subset: IM |
Affiliation:
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Unit of Drug Analysis, Institute of Occupational Health Sciences, Lausanne, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Boranes
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chemistry* Chromatography, Gas Humans Indicators and Reagents / chemistry Methanol / chemistry* Methylation Methylphenidate / analogs & derivatives*, metabolism, urine Temperature |
| Chemical | |
Reg. No./Substance:
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0/Boranes; 0/Indicators and Reagents; 113-45-1/Methylphenidate; 19395-41-6/ritalinic acid; 67-56-1/Methanol; 7637-07-2/boron trifluoride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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