| A safe and practical procedure for global deprotection of oligoribonucleotides. | |
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MedLine Citation:
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PMID: 20670035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report a practical global deprotection of RNA 2'-O-tert-butyldimethylsilyl (TBS) ethers using commercially available aqueous NH(4)F. The procedure is applicable to both 96-well plate format and large-scale production of RNA. This improved procedure provides a safe, mild, and cost-effective alternative to highly hazardous Et(3)N x 3 HF, a reagent commonly used in the routine synthesis of RNA. |
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Authors:
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Daniel Zewge; Francis Gosselin; Rick Sidler; Lisa DiMichele; Raymond J Cvetovich |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of organic chemistry Volume: 75 ISSN: 1520-6904 ISO Abbreviation: J. Org. Chem. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-30 Completed Date: 2010-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985193R Medline TA: J Org Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5305-7 Citation Subset: IM |
Affiliation:
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Department of Process Research, Merck Research Laboratories, P.O. Box 2000, Rahway, New Jersey 07065, USA. daniel_zewge@merck.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Fluorides / chemistry Oligoribonucleotides / chemistry* Quaternary Ammonium Compounds / chemistry Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Fluorides; 0/Oligoribonucleotides; 0/Quaternary Ammonium Compounds; 12125-01-8/ammonium fluoride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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