| s-SHIP promoter expression marks activated stem cells in developing mouse mammary tissue. | |
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MedLine Citation:
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PMID: 20810647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammary stem cells (MaSCs) play critical roles in normal development and perhaps tumorigenesis of the mammary gland. Using combined cell markers, adult MaSCs have been enriched in a basal cell population, but the exact identity of MaSCs remains unknown. We used the s-SHIP promoter to tag presumptive stem cells with GFP in the embryos of a transgenic mouse model. Here we show, in postnatal mammary gland development, that GFP(+) cap cells in puberty and basal alveolar bud cells in pregnancy each exhibit self-renewal and regenerative capabilities for all mammary epithelial cells of a new functional mammary gland upon transplantation. Single GFP(+) cells can regenerate the mammary epithelial network. GFP(+) mammary epithelial cells are p63(+), CD24(mod), CD49f(high), and CD29(high); are actively proliferating; and express s-SHIP mRNA. Overall, our results identify the activated MaSC population in vivo at the forefront of rapidly developing terminal end buds (puberty) and alveolar buds (pregnancy) in the mammary gland. In addition, GFP(+) basal cells are expanded in MMTV-Wnt1 breast tumors but not in ErbB2 tumors. These results enable MaSC in situ identification and isolation via a consistent single parameter using a new mouse model with applications for further analyses of normal and potential cancer stem cells. |
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Authors:
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Lixia Bai; Larry R Rohrschneider |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genes & development Volume: 24 ISSN: 1549-5477 ISO Abbreviation: Genes Dev. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-02 Completed Date: 2010-09-14 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 8711660 Medline TA: Genes Dev Country: United States |
Other Details:
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Languages: eng Pagination: 1882-92 Citation Subset: IM |
Affiliation:
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Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breast Neoplasms / physiopathology Cell Differentiation Female Gene Expression Regulation, Developmental* Gene Expression Regulation, Neoplastic Green Fluorescent Proteins / genetics, metabolism Mammary Glands, Animal / cytology, metabolism* Mice Mice, Transgenic Phosphoric Monoester Hydrolases / genetics* Pregnancy Promoter Regions, Genetic / genetics* Stem Cells / cytology, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA131425-03/CA/NCI NIH HHS; R01 CA40987/CA/NCI NIH HHS; R56 CA123360/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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147336-22-9/Green Fluorescent Proteins; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.56/inositol-1,4,5-trisphosphate 5-phosphatase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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