| The routine measurement of platelet volume: a comparison of light-scattering and aperture-impedance technologies. | |
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MedLine Citation:
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PMID: 3930129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of dipotassium ethylenediaminetetra-acetic acid (EDTA) on platelet count and mean volume (MPV) was evaluated using two routine measurement systems, a Coulter S Plus (Phase 1) (S+) and a Technicon H6000 (H6000). In normal subjects (n = 29) MPV increased by 17% during 39 h storage in EDTA when measured by the S+. In contrast MPV decreased by 22% when measured by the H6000. MPV differences of up to 40% were observed between the two systems. Concomitant platelet counts, in both systems, changed by less than 4%. A mathematical model of the variation of MPV with storage time was constructed, enabling experimental results to be extrapolated, with accuracy, to time zero (MPV0). The H6000 average MPV0 was significantly larger than the S+ average MPV0. Using the anticoagulant sodium citrate and prostaglandin E1 (NaCitrate-PGE1) there were no significant changes in MPV measured by the S+ during 7 h storage, although a linear decrease in platelet count was observed. A decrease in H6000 MPV was observed whether the blood was stored in EDTA or NaCitrate-PGE1. Methodology, anti-coagulation and storage time all influence MPV. Until these determinants are standardised the clinical value of MPV cannot be assessed. |
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Authors:
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E A Trowbridge; D M Reardon; D Hutchinson; C Pickering |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical physics and physiological measurement : an official journal of the Hospital Physicists' Association, Deutsche Gesellschaft für Medizinische Physik and the European Federation of Organisations for Medical Physics Volume: 6 ISSN: 0143-0815 ISO Abbreviation: Clin Phys Physiol Meas Publication Date: 1985 Aug |
Date Detail:
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Created Date: 1985-11-06 Completed Date: 1985-11-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8209031 Medline TA: Clin Phys Physiol Meas Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 221-38 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood Platelets
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analysis* Citrates / pharmacology Citric Acid Edetic Acid / pharmacology Humans Light Models, Biological Platelet Count* Platelet Function Tests / methods* Scattering, Radiation |
| Chemical | |
Reg. No./Substance:
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0/Citrates; 60-00-4/Edetic Acid; 77-92-9/Citric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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