Document Detail

The route of passive chloride movement across amphibian skin: localization and regulatory mechanisms.
MedLine Citation:
PMID:  12421536     Owner:  NLM     Status:  MEDLINE    
Transepithelial Cl(-) conductance (G(Cl)) in amphibian skin can be activated in several species by serosa positive potentials. Mitochondria-rich cells (MRC) or tight junctions (TJ) between the epithelial cells are possible sites for this pathway. The properties and the techniques used to investigate this pathway are reviewed in the present paper. In situ techniques are preferable, since specific properties of the MRC are apparently not maintained in isolated cells. Volume measurements and electronprobe microanalysis of intracellular ions suggest the localization of voltage-activated G(Cl) to MRC. G(Cl) correlates poorly with the density of MRC. The vibrating voltage probe allows quantitative correlation of the local Cl(-) current through morphologically identified structures and the transepithelial Cl(-) current. Our analysis shows that 80% of the voltage-activated Cl(-) current is accounted for by current through MRC or their immediate vicinity. The activation patterns of this current and the inhibition by the alpha(1)-adrenergic agonist, epinephrine, conform to those of the transepithelial current. However, less than 20% of the MRC are active at a certain moment and the activity is spontaneously variable with time. The molecular nature of this pathway, physiological control mechanisms and their relation to the temporal activity of MRC remain to be studied.
Wolfram Nagel; Petra Somieski; Uri Katz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1566     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-11-07     Completed Date:  2003-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  44-54     Citation Subset:  IM    
Physiologisches Institut, Universität München, Schillerstr. 44, München, Germany.
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MeSH Terms
Binding Sites
Biological Transport / drug effects
Cells, Cultured
Chlorides / analysis,  chemistry,  metabolism*
Electric Conductivity
Electric Impedance
Electron Probe Microanalysis
Epinephrine / pharmacology
Epithelium / chemistry,  metabolism
Isotonic Solutions
Patch-Clamp Techniques
Skin / chemistry,  cytology,  metabolism*
Sodium Chloride / metabolism
Reg. No./Substance:
0/Chlorides; 0/Isotonic Solutions; 51-43-4/Epinephrine; 7647-14-5/Sodium Chloride; 8026-10-6/Ringer's solution

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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