| The roles of poly(ADP-ribose)-metabolizing enzymes in alkylation-induced cell death. | |
| | |
MedLine Citation:
|
PMID: 18202825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Poly(ADP-ribose) (PAR) has been identified as a DNA damage-inducible cell death signal upstream of apoptosis-inducing factor (AIF). PAR causes the translocation of AIF from mitochondria to the nucleus and triggers cell death. In living cells, PAR molecules are subject to dynamic changes pending on internal and external stress factors. Using RNA interference (RNAi), we determined the roles of poly(ADP-ribose) polymerases-1 and -2 (PARP-1, PARP-2) and poly(ADP-ribose) glycohydrolase (PARG), the key enzymes configuring PAR molecules, in cell death induced by an alkylating agent. We found that PARP-1, but not PARP-2 and PARG, contributed to alkylation-induced cell death. Likewise, AIF translocation was only affected by PARP-1. PARP-1 seems to play a major role configuring PAR as a death signal involving AIF translocation regardless of the death pathway involved. |
| | |
Authors:
|
O Cohausz; C Blenn; M Malanga; F R Althaus |
Related Documents
:
|
18563705 - Regulatory changes of apoptotic factors in the bovine corpus luteum after induced luteo... 15075075 - Use of a novel histone deacetylase inhibitor to induce apoptosis in cell lines of acute... 17003475 - Alpha-1 antitrypsin inhibits caspase-3 activity, preventing lung endothelial cell apopt... 17039455 - Induction of cell apoptosis in 3t3-l1 pre-adipocytes by flavonoids is associated with t... 17632405 - Regulation of t-cell immunity by t-cell immunoglobulin and mucin domain proteins. 17661345 - Ifn-gamma-induced ido and wrs expression in microglia is differentially regulated by il-4. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cellular and molecular life sciences : CMLS Volume: 65 ISSN: 1420-682X ISO Abbreviation: Cell. Mol. Life Sci. Publication Date: 2008 Feb |
Date Detail:
|
Created Date: 2008-02-22 Completed Date: 2008-05-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9705402 Medline TA: Cell Mol Life Sci Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 644-55 Citation Subset: IM |
Affiliation:
|
Institute of Pharmacology and Toxicology, University of Zurich-Vetsuisse, Winterthurerstrasse 260, 8057, Zurich, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkylation Animals Apoptosis / physiology Cell Death / physiology* DNA Damage Fibroblasts / enzymology Hela Cells Humans Kinetics Mice Poly Adenosine Diphosphate Ribose / metabolism* Poly(ADP-ribose) Polymerases / genetics, metabolism* Polymerase Chain Reaction RNA, Small Interfering / genetics |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Small Interfering; 26656-46-2/Poly Adenosine Diphosphate Ribose; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular motors: not quite like clockwork.
Next Document: The magic of the hypoxia-signaling cascade.