Document Detail


The roles of aerobic exercise training and suppression IL-6 gene expression by RNA interference in the development of insulin resistance.
MedLine Citation:
PMID:  23294974     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To demonstrate the hypothesis that aerobic exercise training inhibits the development of insulin resistance through IL-6 and probe into the possible molecular mechanism about it. METHODS: Rats were raised with high-fat diets for 8weeks to develop insulin resistance, and glucose infusion rates (GIRs) were determined by hyperinsulinemic-euglycemic clamping to confirm the development of insulin resistance. Aerobic exercise training (the speed and duration time in the first week were respectively 16m/min and 50min, and speed increased 1m/min and duration time increased 5min every week following it) and/or IL-6shRNA plasmid injection (rats received IL-6shRNA injection via the tail vein every two weeks) were adopted during the development of insulin resistance. The serum IL-6, leptin, adiponectin, fasting blood glucose, fasting serum insulin, GIR, IL-6 gene expression levels, p-p38 in various tissues and p-STAT3/t-STAT3 ratio in the liver were measured. RESULTS: Rats fed with high-fat diets for 8weeks were developed insulin resistance and the IL-6mRNA levels of IL-6shRNA injection groups in various tissues were significantly lower than those of control group (P<0.05), respectively. The development of insulin resistance in exercise rats significantly decreased, however, compared with that, the GIR of exercise rats injected by IL-6shRNA was lower (P<0.05). The IL-6mRNA levels were highest in the fat tissue and lowest in the skeletal muscles in all the rats. The serum adiponectin levels decreased (P<0.05) following the development of insulin resistance, and it increased (P<0.05) when the rats were intervened by aerobic exercise training for 8weeks at the same time. However, there were not significant differences when serum leptin concentrations were compared (P>0.05). The p-p38 significantly increased in the rats fed with high-fat diets, however, p-p38 of the exercise high-fat diets rats in the liver and fat tissues significantly decreased than that (P<0.05). The changes of p-p38 in exercise rats injected by IL-6shRNA were irregular. The activation of STAT3 in the liver significantly increased (P<0.05) following the development of insulin resistance, and it decreased (P<0.05) when the rats were intervened by aerobic exercise training for 8weeks at the same time, and the gene silencing of IL-6 did not have effects on the activation of STAT3 in the liver (P>0.05). CONCLUSIONS: In conclusion, aerobic exercise training prevented the development of insulin resistance through IL-6 to a certain degree. The gene expression and secretion of IL-6 could inhibit the development of insulin resistance. The mechanism of the effects were possibly related with elevating the levels of serum adiponectin, and/or inhibiting the activation of STAT3 in the liver and p38MAPK in the skeletal muscles, liver and fat tissues.
Authors:
Hui Tang; Min-Hao Xie; Yu Lei; Liang Zhou; Yu-Ping Xu; Jian-Guang Cai
Related Documents :
25028994 - Inflammatory markers and adipocytokine responses to exercise training and detraining in...
1553454 - Hormonal and plasma volume alterations following endurance exercise. a brief review.
24290114 - Characteristics and outcomes of patients who achieve high workload (≥10 metabolic equ...
23672384 - Fatigue-induced alterations of static and dynamic postural control in athletes with a h...
14729724 - Influence of expiratory loading and hyperinflation on cardiac output during exercise.
16091494 - Leptin and adiponectin responses in overweight inactive elderly following resistance tr...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-4
Journal Detail:
Title:  Cytokine     Volume:  -     ISSN:  1096-0023     ISO Abbreviation:  Cytokine     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Physical Education, Hunan University of Science and Technology, Xiangtan, Hunan Province, China. Electronic address: tanghui19730711@163.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Association of TNF-? polymorphism rs1800629 with multisomatoform disorder in a group of German patie...
Next Document:  The influence of a TNF gene polymorphism on the severity of rheumatoid arthritis in the Brazilian Am...