| The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction. | |
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MedLine Citation:
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PMID: 20167873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Disruption of the intestinal barrier is a causal factor in the development of alcoholic endotoxemia and hepatitis. This study was undertaken to determine whether zinc deficiency is related to the deleterious effects of alcohol on the intestinal barrier. Mice were pair fed an alcohol or isocaloric liquid diet for 4 wk, and hepatitis was detected in association with elevated blood endotoxin level. Alcohol exposure significantly increased the permeability of the ileum but did not affect the barrier function of the duodenum or jejunum. Reduction of tight-junction proteins at the ileal epithelium was detected in alcohol-fed mice although alcohol exposure did not cause apparent histopathological changes. Alcohol exposure significantly reduced the ileal zinc concentration in association with accumulation of reactive oxygen species. Caco-2 cell culture demonstrated that alcohol exposure increases the intracellular free zinc because of oxidative stress. Zinc deprivation caused epithelial barrier disruption in association with disassembling of tight junction proteins in the Caco-2 monolayer cells. Furthermore, minor zinc deprivation exaggerated the deleterious effect of alcohol on the epithelial barrier. In conclusion, epithelial barrier dysfunction in the distal small intestine plays an important role in alcohol-induced gut leakiness, and zinc deficiency attributable to oxidative stress may interfere with the intestinal barrier function by a direct action on tight junction proteins or by sensitizing to the effects of alcohol. |
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Authors:
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Wei Zhong; Craig J McClain; Matthew Cave; Y James Kang; Zhanxiang Zhou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-18 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 298 ISSN: 1522-1547 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-20 Completed Date: 2010-05-06 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G625-33 Citation Subset: IM |
Affiliation:
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China Agricultural University, Beijing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Drinking
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physiopathology* Animals Caco-2 Cells Ethanol / pharmacology Humans Ileum / physiology* Intestines / drug effects* Liver Diseases, Alcoholic / pathology, physiopathology Male Mice Mice, Inbred C57BL Oxidative Stress Permeability / drug effects Reactive Oxygen Species / metabolism Tight Junctions / metabolism Zinc / deficiency* |
| Grant Support | |
ID/Acronym/Agency:
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K23 AA018399-02/AA/NIAAA NIH HHS; K23 AA018399-03/AA/NIAAA NIH HHS; R01 AA018016-04/AA/NIAAA NIH HHS; R01 AA018869-03/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 64-17-5/Ethanol; 7440-66-6/Zinc |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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