Document Detail


The role of wild-type p53 in the differentiation of primary hemopoietic and muscle cells.
MedLine Citation:
PMID:  10523864     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments previously performed on 32D and C2C12 cell lines indicated that wild type p53 (wtp53) protein has a role in granulocyte and myotube differentiation. Since these are immortal cells, we asked whether the inhibition of differentiation induced by the expression of dominant-negative p53 (dnp53) proteins was dependent on the immortalization-determined microenvironment. Thus, we evaluated the effects produced by interfering with the endogenous p53 gene in murine primary hemopoietic and muscle cells. Expression of dnp53 protein reduced the differentiation of bone marrow cells into granulocytes and macrophages. Moreover, p53 activation was measurable during the differentiation process of primary myoblasts, while interference with this activation led to a consistent slow down of terminal differentiation. Since the impairment of the differentiation was not accompanied by alterations in the cell cycle withdrawal and in the rate of apoptosis which are coupled with these types of differentiation, the data here reported support a specific role for p53 in the differentiation process. However, the difference in the intensity of inhibition between immortal and primary cells, i. e., complete versus slow down, respectively, suggests that the immortalization process might render the cells more sensitive to the loss of wtp53 activity for the differentiation process.
Authors:
G Mazzaro; G Bossi; S Coen; A Sacchi; S Soddu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  18     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-19     Completed Date:  1999-11-19     Revised Date:  2012-02-22    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  5831-5     Citation Subset:  IM    
Affiliation:
Molecular Oncogenesis Laboratory, Regina Elena Cancer Institute, C.R. S., Via delle Messi d'Oro 156, 00158 Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells / cytology
Cell Differentiation / physiology
Cell Division / physiology
Cell Line
Cell Line, Transformed
Cell Survival / physiology
Coculture Techniques
Hematopoietic Stem Cells / cytology*
Mice
Muscles / cytology*
Stem Cells / cytology
Transcription, Genetic
Tumor Suppressor Protein p53 / physiology*
Grant Support
ID/Acronym/Agency:
857//Telethon
Chemical
Reg. No./Substance:
0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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