Document Detail

The role of the vacB gene in the pathogenesis of Brucella abortus.
MedLine Citation:
PMID:  17306588     Owner:  NLM     Status:  MEDLINE    
Brucella species are important zoonotic pathogens affecting a wide variety of mammals. Therefore, the identification of new Brucella virulence factors is of great interest in understanding bacterial pathogenesis and immune evasion. In this study, we have identified Brucella abortus vacB gene that presents 2343 nucleotides and 781 amino acids and it shows 39% identity with Shigella flexneri vacB gene that encodes an exoribonuclease RNase R involved in bacterial virulence. Further, we have inactivated Brucella vacB by gene replacement strategy generating a deletion mutant strain. In order to test the role of Brucella vacB in pathogenesis, BALB/c and interferon regulatory factor-1 (IRF-1) knockout (KO) mice received Brucella vacB mutant, the virulent parental strain 2308 or the vaccine strain RB51 and the bacterial CFU numbers in spleens and mous survival were monitored. Our results demonstrated that the B. abortus DeltavacB mutant and the wild type strain 2308 showed similar CFU numbers in BALB/c mice. Additionally, IRF-1 KO mice that received either the vacB mutant or S2308 strain died in 12-14 days postinfection; in contrast, all animals that received the RB51 vaccine strain survived for 30 days postinoculation. In summary, this study reports that the vacB gene in B. abortus has no impact on bacterial pathogenesis.
Anderson Miyoshi; Gracia M S Rosinha; Ilana L B C Camargo; Cyntia M C Trant; Fernanda C Cardoso; Vasco Azevedo; Sergio C Oliveira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-04
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  9     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-09     Completed Date:  2007-05-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  375-81     Citation Subset:  IM    
Department of General Biology, Federal University of Minas Gerais, Minas Gerais, Pampulha, Belo Horizonte, MG, Brazil.
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / chemistry,  genetics*,  metabolism
Base Sequence
Brucella Vaccine / administration & dosage
Brucella abortus / genetics,  metabolism,  pathogenicity*
Brucellosis / immunology,  microbiology,  mortality,  prevention & control
Exoribonucleases / chemistry,  genetics*,  metabolism
Gene Deletion
Interferon Regulatory Factor-1 / genetics
Mice, Inbred BALB C
Mice, Knockout
Molecular Sequence Data
Sequence Analysis, DNA
Spleen / microbiology
Reg. No./Substance:
0/Bacterial Proteins; 0/Brucella Vaccine; 0/Interferon Regulatory Factor-1; EC 3.1.-/Exoribonucleases; EC 3.1.27.-/ribonuclease R

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