| The role of the vacB gene in the pathogenesis of Brucella abortus. | |
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MedLine Citation:
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PMID: 17306588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brucella species are important zoonotic pathogens affecting a wide variety of mammals. Therefore, the identification of new Brucella virulence factors is of great interest in understanding bacterial pathogenesis and immune evasion. In this study, we have identified Brucella abortus vacB gene that presents 2343 nucleotides and 781 amino acids and it shows 39% identity with Shigella flexneri vacB gene that encodes an exoribonuclease RNase R involved in bacterial virulence. Further, we have inactivated Brucella vacB by gene replacement strategy generating a deletion mutant strain. In order to test the role of Brucella vacB in pathogenesis, BALB/c and interferon regulatory factor-1 (IRF-1) knockout (KO) mice received Brucella vacB mutant, the virulent parental strain 2308 or the vaccine strain RB51 and the bacterial CFU numbers in spleens and mous survival were monitored. Our results demonstrated that the B. abortus DeltavacB mutant and the wild type strain 2308 showed similar CFU numbers in BALB/c mice. Additionally, IRF-1 KO mice that received either the vacB mutant or S2308 strain died in 12-14 days postinfection; in contrast, all animals that received the RB51 vaccine strain survived for 30 days postinoculation. In summary, this study reports that the vacB gene in B. abortus has no impact on bacterial pathogenesis. |
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Authors:
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Anderson Miyoshi; Gracia M S Rosinha; Ilana L B C Camargo; Cyntia M C Trant; Fernanda C Cardoso; Vasco Azevedo; Sergio C Oliveira |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-04 |
Journal Detail:
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Title: Microbes and infection / Institut Pasteur Volume: 9 ISSN: 1286-4579 ISO Abbreviation: Microbes Infect. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-09 Completed Date: 2007-05-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883508 Medline TA: Microbes Infect Country: France |
Other Details:
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Languages: eng Pagination: 375-81 Citation Subset: IM |
Affiliation:
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Department of General Biology, Federal University of Minas Gerais, Minas Gerais, Pampulha, Belo Horizonte, MG, Brazil. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF174645 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Bacterial Proteins / chemistry, genetics*, metabolism Base Sequence Brucella Vaccine / administration & dosage Brucella abortus / genetics, metabolism, pathogenicity* Brucellosis / immunology, microbiology, mortality, prevention & control Exoribonucleases / chemistry, genetics*, metabolism Female Gene Deletion Humans Interferon Regulatory Factor-1 / genetics Mice Mice, Inbred BALB C Mice, Knockout Molecular Sequence Data Sequence Analysis, DNA Spleen / microbiology Virulence |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Brucella Vaccine; 0/Interferon Regulatory Factor-1; EC 3.1.-/Exoribonucleases; EC 3.1.27.-/ribonuclease R |
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