Document Detail


A role for upstream binding factor in organizing ribosomal gene chromatin.
MedLine Citation:
PMID:  16626289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human ribosomal genes are located in NORs (nucleolar organizer regions) on the short arms of acrocentric chromosomes. During metaphase, previously active NORs appear as prominent chromosomal features termed secondary constrictions, which are achromatic in chromosome banding and positive in silver staining. The architectural RNA polymerase I transcription factor UBF (upstream binding factor) binds extensively across the ribosomal gene repeat throughout the cell cycle. Evidence that UBF underpins NOR structure is provided by an examination of cell lines in which large arrays of a heterologous UBF binding sequences are integrated at ectopic sites on human chromosomes. These arrays efficiently recruit UBF even to sites outside the nucleolus, and during metaphase form novel silver-stainable secondary constrictions, termed pseudo-NORs, that are morphologically similar to NORs.
Authors:
Jane E Wright; Christine Mais; José-Luis Prieto; Brian McStay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Biochemical Society symposium     Volume:  -     ISSN:  0067-8694     ISO Abbreviation:  Biochem. Soc. Symp.     Publication Date:  2006  
Date Detail:
Created Date:  2006-04-21     Completed Date:  2006-05-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506896     Medline TA:  Biochem Soc Symp     Country:  England    
Other Details:
Languages:  eng     Pagination:  77-84     Citation Subset:  IM    
Affiliation:
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK.
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MeSH Terms
Descriptor/Qualifier:
Binding Sites / genetics
Chromatin / genetics*,  metabolism*
DNA, Ribosomal / genetics,  metabolism
Humans
Nucleolus Organizer Region / genetics*,  metabolism*
Pol1 Transcription Initiation Complex Proteins / genetics*,  metabolism*
Protein Binding
Ribosomes / genetics*,  metabolism*
Chemical
Reg. No./Substance:
0/Chromatin; 0/DNA, Ribosomal; 0/Pol1 Transcription Initiation Complex Proteins; 0/transcription factor UBF

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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