| The role of uncoupling protein 2 in the apoptosis induced by free fatty acid in rat cardiomyocytes. | |
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MedLine Citation:
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PMID: 20010438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The apoptotic loss of cardiomyocytes contributes to numerous cardiovascular disorders. Evidence suggests that free fatty acids induce cellular apoptosis, and recent studies have shown that free fatty acids dramatically elevate mRNA levels of uncoupling protein 2 (UCP2) in some cell lines. In this study, we investigated the possibility that free fatty acids induce the expression of UCP2 through the peroxisome proliferator-activated receptor pathway, thereby increasing cell apoptosis in adult rat cardiomyocytes. Primary cultured adult rat cardiomyocytes exposed to free fatty acids exhibited a dose-dependent increase in apoptosis. Quantitative real-time reverse transcription-polymerase chain reaction and Western blotting showed significant increases in the level of UCP2 expression at 6, 12, and at 24 hours after treatment of adult rat cardiomyocytes with free fatty acids. Expression of UCP2 was suppressed with RNA interference, and knockdown of UCP2 attenuated free fatty acid-induced apoptosis in the cardiomyocytes. In summary, free fatty acids induced UCP2 expression through peroxisome proliferator-activated receptor alpha in adult rat cardiomyocytes. |
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Authors:
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Nan Li; Jiang Wang; Feng Gao; Ying Tian; Rong Song; Shan-jun Zhu |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cardiovascular pharmacology Volume: 55 ISSN: 1533-4023 ISO Abbreviation: J. Cardiovasc. Pharmacol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-24 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902492 Medline TA: J Cardiovasc Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 161-7 Citation Subset: IM |
Affiliation:
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Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / physiology* Cell Line, Tumor Cells, Cultured Fatty Acids, Nonesterified / physiology* Female Ion Channels / biosynthesis, physiology* Mitochondrial Proteins / biosynthesis, physiology* Myocytes, Cardiac / cytology, metabolism, physiology Peroxisome Proliferator-Activated Receptors / physiology Rats Rats, Sprague-Dawley Signal Transduction / physiology |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Ion Channels; 0/Mitochondrial Proteins; 0/Peroxisome Proliferator-Activated Receptors; 0/mitochondrial uncoupling protein 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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