Document Detail

The role of triiodothyronine in the regulation of synthesis rate and translatable mRNA level of fatty acid synthetase in a preadipocyte cell line.
MedLine Citation:
PMID:  6383478     Owner:  NLM     Status:  MEDLINE    
Triiodothyronine (T3) effects on the activity, rate of synthesis and mRNA content of the key lipogenic enzyme, fatty acid synthetase, were studied in differentiating ob17 preadipocytes cloned from ob/ob mouse epididymal adipose tissue. During differentiation in the presence of insulin, a 6-10-fold increase in both fatty acid synthetase specific activity and synthesis rate were reproducibly observed and occurred concomitantly. The relative synthesis rate exhibited a progressive elevation from 0.5% at confluence to a maximum level of 2% in the presence of insulin. The rate of the enzyme degradation determined by pulse-chase experiments was similar in differentiating cells and insulin-untreated cells of the same age (t 1/2, 40-42 h). Furthermore, the increase in the enzyme synthesis rate was preceded by a progressively elevating amount of mRNA encoding for this protein as detected by translation in a reticulocyte lysate cell-free system. It is thus suggested that the increment in total and neosynthesized fatty acid synthetase in essentially due to an increased enzyme synthesis, reflecting an increased relative content of its specific mRNA. T3 included at a physiological concentration (1.5 nM) in the culture medium enhanced significantly both enzyme synthesis and its specific mRNA. The most important T3 effect was an acceleration of both processes, a stimulation of the mRNA level being detected as early as day 3 post-confluence and maximum at day 5 when the effect on the synthetase synthesis rate and activity began to be enhanced. This suggests that T3 would mainly affect fatty acid synthetase as a pretranslational level.
J Gharbi-Chihi; O Chabaud; J Torresani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  783     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-11-13     Completed Date:  1984-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  26-35     Citation Subset:  IM    
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MeSH Terms
Adipose Tissue / cytology,  enzymology*
Cell Differentiation
Cell Line
Electrophoresis, Polyacrylamide Gel
Fatty Acid Synthetase Complex / genetics*
Insulin / pharmacology
Protein Biosynthesis
RNA, Messenger / metabolism*
Time Factors
Triiodothyronine / pharmacology*
Reg. No./Substance:
0/RNA, Messenger; 11061-68-0/Insulin; 6893-02-3/Triiodothyronine; EC 6.-/Fatty Acid Synthetase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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