Document Detail

The role of trefoil factor family in apparently healthy subjects administrated gastroprotective agents for the primary prevention of gastrointestinal injuries from low-dose acetylsalicylic acid: a preliminary study.
Jump to Full Text
MedLine Citation:
PMID:  21980231     Owner:  NLM     Status:  PubMed-not-MEDLINE    
It is well-known that acetylsalicylic acid induces gastrointestinal complication. Recently, trefoil factor family has been reported as a mucosal protective factor. We focused on trefoil factor family as one of defensive system for gastrointestinal injuries. The aim of this trial was to evaluate trefoil factor family levels in the serum of healthy subjects with low-dose acetylsalicylic acid. Low-dose acetylsalicylic acid with placebo or proton pump inhibitor or rebamipide were administered in 30 healthy subjects. Transnasal endoscopy was performed at 0, 24 h, 3 and 7 day. Changing of trefoil factor family (1,2,3) and numbers of gastric injuries were evaluated. The numbers of gastric injuries were significantly increased in the placebo group at 3 and 7 days. Injuries in the proton pump inhibitor group were not induced, in the rebamipide group were slightly induced. Trefoil factor family level in the placebo group were decreased in 3 and 7 days compared with prior to starting the trial. Trefoil factor family may have an important association with acetylsalicylic acid-induced gastrointestinal damage. Proton pump inhibitor and rebamipide prevented low-dose acetylsalicylic acid-induced gastrointestinal complications compared with the placebo group.
Takashi Kawai; Yu Takagi; Mari Fukuzawa; Tetsuya Yamagishi; Shinya Goto
Publication Detail:
Type:  Journal Article     Date:  2011-08-25
Journal Detail:
Title:  Journal of clinical biochemistry and nutrition     Volume:  49     ISSN:  1880-5086     ISO Abbreviation:  J Clin Biochem Nutr     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-10-07     Completed Date:  2011-11-10     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8700907     Medline TA:  J Clin Biochem Nutr     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  136-40     Citation Subset:  -    
Endoscopy Center, Tokyo Medical University Hospital, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Clin Biochem Nutr
Journal ID (publisher-id): JCBN
ISSN: 0912-0009
ISSN: 1880-5086
Publisher: the Society for Free Radical Research Japan, Kyoto, Japan
Article Information
Download PDF
Copyright © 2011 JCBN
Received Day: 11 Month: 1 Year: 2011
Accepted Day: 9 Month: 2 Year: 2011
Print publication date: Month: 9 Year: 2011
Electronic publication date: Day: 25 Month: 8 Year: 2011
Volume: 49 Issue: 2
First Page: 136 Last Page: 140
ID: 3171679
PubMed Id: 21980231
Publisher Id: jcbn11-10
DOI: 10.3164/jcbn.11-10

The role of trefoil factor family in apparently healthy subjects administrated gastroprotective agents for the primary prevention of gastrointestinal injuries from low-dose acetylsalicylic acid: a preliminary study
Takashi Kawai1*
Yu Takagi2
Mari Fukuzawa1
Tetsuya Yamagishi1
Shinya Goto3
1Endoscopy Center, Tokyo Medical University Hospital, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan
23rd Department of Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan
3Department of Medicine (Cardiology) and Metabolic Disease Center, Tokai University School of Medicine, Kanagawa 259-1193, Japan
Correspondence: *To whom correspondence should be addressed. E-mail:


Atherosclerotic diseases including coronary heart disease and cardiovascular events are associated with high frequencies of morbidity and mortality in developed populations.(1) Administration of low-dose acetylsalicylic acid (ASA) has been recommended for the primary and secondary prevention of these diseases.(23) Indeed, observational registry demonstrated that approximately 70–80% of patients with a high risk of atherothrombosis were given low-dose ASA for the prevention of future vascular events.(4)

On the other hand, it is well-known that low-dose ASA induces gastrointestinal (GI) complications. In a multinational prospective study of 187 patients receiving low-dose ASA for cardiovascular disease prophylaxis, the prevalence of endoscopically-detected peptic ulcers was 10.7% [95% confidence interval (CI); 6.3–15.1] in the US 5). There was also a report on low-dose ASA-induced GI complications for a Japanese population. Nema et al.(6) demonstrated that mucosal injuries were found in 61.4% of low-dose ASA users; the low-dose ASA users had more mucosal injuries than the non-users (p<0.0001). The frequency of mucosal injuries was not associated with the duration of ASA treatment. Also, there were several reports on the preventive effect of anti-acid agents for low-dose ASA-induced GI complications.

Proton pump inhibitor (PPI) was effective at reducing the risk of endoscopic duodenal ulcer (RR = 0.44, 95% CI; 0.32–0.74) and gastric ulcer (RR = 0.40, 95% CI; 0.32–0.51).(7) Shiotani et al.(8) reported that taking PPI [adjusted odds ratio (OR): 0.09; 95% CI: 0.02–0.39] was significantly associated with peptic ulcer. Prostaglandin (PG) analogue and inducer include misoprostol and rebamipide, and they are cytoprotective agents. Misoprostol is well-known reducing serious GI complications in patients with rheumatoid arthritis taking NSAIDs.(9) There is a randomized controlled trial compared with misoprostol and rebamipide for patients with NSAIDs-induced GI complications. It was resulted that the incidence of peptic ulcer was 4.4% in the misoprostol group and 4.5% in the rebamipide group.(10) Ono et al.(11) reported that rebamipide prevent low-dose aspirin-induced gastric injury in healthy subject.

In Europe it was reported that PPI was prescribed in 99.4% cases for prevention of low-dose ASA-induced GI complications.(12) On the other hand, in Japan cytoprotective agents (ex. rebamipide) were widely used for prophylaxis of gastrointestinal complication induced by low-dose ASA. Actually PPI and cytoprotective agents were prescribed equally for prophylaxis of gastrointestinal complication induced by low-dose ASA.(13)

In our previous report, low-dose ASA-induced GI complications for healthy subjects were strongly induced at 3 days and were reduced at 7 days.(14) This phenomenon was concluded as adaptation. The mechanism of adaptation is not yet well understood, therefore we conducted this study to clarify the phenomenon by measuring trefoil factor family (TFF).

Recently, TFF has been reported to be a mucosal protective factor. TFF is a mucin-associated protein involved in the maintenance of mucosal barrier and restitution of lining epithelial cells.(15) TFF were categorized into three family members, TFF1, 2, and 3. TFF are a family of short peptides, rich in disulfide bonds that form intramolecular loops.(16) TFF are up-regulated in the surface epithelial cells at the margin of gastric ulcer.(17) We focused on TFF as one candidate defensive system for ASA-induced GI complications.

The aim of this trial was to evaluate the level of TFF in the serum for healthy subjects on low-dose ASA, ASA plus PPI, and ASA plus rebamipide, and the preventive effect of PPI and rebamipide.

Materials and Methods

A randomized, double-blind, placebo-controlled trial comparing placebo, PPI and rebamipide were performed in 30 healthy subjects. Ten were assigned as the PPI group, ten were as the placebo group, and ten were as the rebamipide group. The study protocol was approved by the Ethics Committees of Tokyo Medical University Hospital, and written informed consent was obtained from all subjects.

Inclusion and exclusion criteria

Inclusion criteria were i) lack of gastric conditions such as bleeding or ulcer on endoscopy, and ii) H. pylori negativity on 13C urea breath test. Subjects who were taking other medication were excluded.

Study design

The study design was as shown in Fig. 1. The thirty healthy subjects were divided into three groups: those taking ASA 100 mg with placebo, omeprazole 20 mg, or rebamipide 300 mg. The groups with drug administration received their dose three times daily for 7 consecutive days. Transnasal endoscopy was performed at 0 and 24 h on the first day, and again on the third and seventh days.


Subjects were recruited for the treatment sequences in a random fashion according to a randomization schedule for the treatment period. A randomization number that was associated with a specific treatment, either rebamipide, omeprazole, or placebo, was assigned to each subject in the study. Randomized numbers were generated by SAS program.


The primary endpoint was to evaluate short-term changes of gastric mucosa in healthy subjects who took low-dose ASA with placebo, omeprazole, and rebamipide. The secondary endpoint was to evaluate short-term changes of TFF (TFF1, 2, and 3) levels in serum.

Evaluation criteria

The categories of injury were erythema, erosions, petechia, and ulcers. Erythema was defined as a region clearly redder than surrounding mucosa, erosion as a region with mucosal deficit, petechia as a bleeding region without mucosal deficit, and ulcer was defined as greater than 5 mm. Gastric mucosal injuries detected on endoscopy were calculated among numbers of erosions, erythema, petechia, and ulcers. All GI injuries were counted in each time course.

The number of erythema, erosions, petechia and ulcers were counted to evaluate low-dose ASA-induced GI injuries compared among the placebo, omeprazole, and rebamipide group. The number of erythema, erosions, petechia were evaluated as changing their numbers compared with before starting study and after (after had three points, 24 h, 3 days, and 7 days).

Endoscopic evaluation was done by two endoscopists blinded for subject number and groups. If the result was different, re-evaluation and discussion were done.

Measurement of serum and TFF1, 2, and 3 levels in the serum

Serum samples were obtained for all subjects at 0, 24 h on the first day, 3rd and 7th days after starting this trial. TFF1, TFF2, and TFF3 levels in the gastric juice and serum were measured by ELISA as described recently.(18,19) All measurements were performed in duplicate within the same assay run.

Evaluation of adverse events

The following GI symptoms and complications were to be recorded in the symptom diary for all subjects throughout the study period.

Statistical analysis

Difference of time course in TFF levels were shown as absolute different and 95% confidence index. Findings of p<0.05 were considered significant. Statistical analyses were performed using SAS® version 8.2 (SAS Institute, Cary, NC). Details of the statistical analysis were analyzed by intention-to-treat analysis.


Thirty healthy subjects were enrolled. The mean age of the subjects was 28 ± 4 years. Nineteen were male and eleven were female. In addition, the backgrounds of all subjects who had smoking, drinking, and histology data before starting trial were shown in Table 1. No subjects had H. pylori infection. Thirty healthy subjects were divided into three groups: low-dose ASA plus placebo group, omeprazole, and rebamipide groups.

Short-term change of gastric mucosa by administration of low-dose ASA among the control, placebo, omeprazole, and rebamipide groups

Changing of erosion, erythema, and petechiae among three groups from before starting the trial were shown in Table 2. The number of erosion and erythema were increased in the placebo group at 3 days compared with before starting the trial. Afterward these changes were recovered at 7 days. The number of petechiae was increased in the placebo group at 7 days compared with before starting the trial. The number of erythema was increased in the placebo group at 3 days compared with the omeprazole and the rebamipide groups, 9.6 ± 10.5 vs 1.4 ± 6.8 (p = 0.0611) vs 0.3 ± 4.2 (p = 0.0327). The number of petechiae was increased in the placebo group at 7 days compared with the omeprazole and the rebamipide groups: 8.3 ± 8.8 vs −5.6 ± 23.6 (p = 0.0213) vs −1.6 ± 5.2 (p = 0.0335), respectively.

Evaluation of TFF1, 2, and 3 levels in the serum

TFF levels at 3 day in the placebo group were decreased compared with before taking ASA, and were recovered at 7 days. On the other hand, TFF levels in PPI group were not changed compared with before starting the trial and 3, 7 days. TFF1, 2 levels at 3 day in rebamipide were significantly decreased compared with before taking ASA, and were also recovered at 7 days. (shown in Table 3)

Adverse events

The number of gastrointestinal symptoms or complications was recorded in the symptom diary by all subjects throughout the study period. There were no serious side-effects in three treatment groups.


The action of ASA is derived from irreversible inhibition of the cyclooxygenase-1 enzyme, resulting in the inhibition of thromboxane A2 formation, however the novel effects of ASA mediated by its salicylate moiety complement the role of the acetyl moiety in terms of its platelet inhibitory effect. The action of ASA resulted from an interaction among platelet activation, endothelial inflammation/activation, and oxidative stress.(20) However, they may have contrary effects for GI mucosa.

In this study, it is interesting to note that the peak of erythema and erosion was induced on the 3rd day, and restored on the 7th day in spite of continued administration of ASA (Table 2). Konturek et al.(21) reported about adaptation of aspirin-induced gastric damage. Gastric injury may repeatedly occur and heal as a natural process, but it is not clear what kind of cycle is being repeated. In this study, the healing effect of placebo at 7 days may be resulted by adaptation, and also this action by adaptation may have a close relation to TFF.

TFF is relatively new family peptides which bear the three-loop trefoil domain. They are assumed to play important role in the protection of gastrointestinal mucosa.(22,23) TFF1 is produced mainly in the stomach, in superficial cells of the body and antral mucosa.(22,24) TFF2 is abundant in the mucous neck cells in the body and in the antral gland of the stomach.(22,25) TFF3 is expressed in the goblet cells of lower intestine and its expression in not found in normal gastric mucosa.(17) We also planned the measurement of TFF in gastric mucosa, but this procedure requires the use of biopsy forceps. We must take measurements many times (pre, 24 h, 3, 7 day), so constant use of the forceps cause injury to gastric mucosa. Such injuries could also conceivably be caused by low-dose aspirin as well. We cannot tell if the injury is caused by the forceps or low-dose aspirin. Therefore we used serum TFF in this study.

The relationship between TFF and NSAIDs is still somewhat controversial. Koitabashi et al.(26) reported Indometacin up-regulates TFF2 expression in gastric epithelial cell in vitro. Alderman et al.(27) investigated relationship between TFF1 & TFF2 and adaptation to aspirin in gastric mucosa in vivo. They reported that quantification of TFF1 and TFF2 protein level in adapted group slightly were decreased (but no significant change) than that of control group. Moreover there are no report related serum TFF and mucosal TFF, but transvenous administration of TFF inhibited ethanol induced injury.(28) It is possible that serum TFF is strongly related with mucosal TFF. TFF may possibly play an important role in gastric mucosal damage.

In addition, we investigated the relationship between TFF levels and mucosal damage by low-dose ASA with PPI or rebamipide. Low-dose ASA-induced GI injuries have been prevented in the PPI group, the level of TFF in the PPI group were not significant changed compared with before starting the trial. This was based on the acid secretion inhibitory action of PPI. On the other hand, rebamipide prevented GI injuries in spite of decrease in TFF levels. These results may show that protective effect of rebamipide was not influenced by TFF levels. Suzuki et al.(29) reported that rebamipide significantly suppressed ASA-induced gastric damages by protection of tight-junction. Iijima et al.(30) reported on the output of total gastric mucin was significantly increased by rebamipide. These actions may be one of rebamipide’s GI mucosal protective mechanisms.

Although PPI is the most effective drug for patients with ASA-induced GI complications, it also has limitations, such as poor metabolizer as well as ASA and/or clopidogrel resistance. Some patients are known to have PPI resistance.(31,32) PPI can be classified as having either the phenotype of an extensive metabolizer or that of a poor metabolizer based on the level of CYP2C19 activity.(33,34) Manifestation of a PPI-independent preventive system for GI complications would be useful because there are numerous patients with PPI resistance. A candidate drug with a mechanism different from that of PPI should be developed. More recent interesting report demonstrated that concomitant use of PPI and gastroprotective effects of rebamipide, suggesting that it may be a good choice in aspirin users with gastroduodenal toxicity that is not suppressed by acid suppressants alone.(35)

Total care between cardiovascular events and GI complications are important for patients with atherosclerotic diseases who are taking anti-platelet agents. Moreover, various patients often have different circumstances; therefore it is necessary to prepare various clinical options. Recently, ASA-related small intestinal complication was well discussed. Niwa et al.(36) demonstrated that rebamipide has the preventive effect for NSAID-induced small-intestinal injuries. This result showed the possibility for management of the entire gastrointestinal tract including the small intestinal mucosa by concomitant therapy of PPI + rebamipide.(37) In addition, most patients who take ASA are asymptomatic, even if GI ulcer and bleeding occurs, and there is no option for these patients, except endoscopy testing. However, periodic diagnosis is essential. Recently, transnasal upper-gastrointestinal endoscopy has become popular; we too used this modality in this trial, and no subjects withdrew. Transnasal endoscopy has been developed as an alternative to trans-oral endoscopy to reduce medical costs as well as the risks associated with conscious sedation.(38,39) In addition, transnasal upper GI endoscopy is a comfortable and conventional test that imposes less stress due to sedation, such as respiratory depression, hypotension, paradoxical agitation, and anaphylaxis,(40) and most importantly, imposes less load on the cardiovascular pulmonary system.(41) This modality will become the mainstream method for periodical diagnosis. In this study, not only PPI but also rebamipide showed a preventive effect for low-dose ASA-induced GI complications. It was clear that the action of PPI was by acid suppression without TFF. The role of TFF was reported to be site protective action. TFF is part of the gastric mucosal defensive system.

In conclusion, Administration of low-dose ASA decreased TFF levels in serum. TFF may have an association with ASA-induced GI damage. These relations between GI-mucosal damage and the expression of various TFF, and the search for drugs that have an action for TFF, deserve further investigation.


Prof. Goto has received honoraria and consulting fees from Astellas, AstraZeneca, Bayer, Bristol-Myers Squibb, Daiichi-Sankyo, Eisai, GlaxoSmithKline, Kowa, Novartis, Otsuka, sanofi-aventis, Schering-Plough, and Takeda. Prof. Goto also received research grants from Eisai, Ono, sanofi-aventis, Astra Zeneca, Kowa, and Pfizer within the last three years.

ASA acetylsalicylic acid
CI confidence interval
GI Gastrointestinal
PPI Proton pump inhibitor
TFF trefoil factor family

1. Lopez AD,Mathers CD,Ezzati M,Jamison DT,Murray CJ. Global and regional burden of disease and risk factors, 2001: systemic analysis of population health dataLancetYear: 20063671747175716731270
2. Mehta P. Aspirin in the prophylaxis of coronary artery diseaseCurr Opin CardiolYear: 20021755255812357134
3. Gasparyan AY,Watson T,Lip GY. The role of aspirin in cardiovascular prevention: implications of aspirin resistanceJ Am Coll CardiolYear: 2008511829184318466797
4. Bhatt DL,Steg PG,Ohman EM,et al. REACH Registry Investigators. International prevalence, recognition, and treatment of cardiovascular risk factors in outpatients with atherothrombosisJAMAYear: 200629518018916403930
5. Yeomans ND,Lanas AI,Talley NJ,et al. Prevalence and incidence of gastroduodenal ulcers during treatment with vascular protective dose of aspirinAliment Pharmacol TherYear: 20052279580116225488
6. Nema H,Kato M,Katsurada T,et al. Endoscopic survey of low-dose-aspirin-induced gastroduodenal mucosal injuries in patients with ischemic heart diseaseJ Gastroenterol HepatolYear: 200823S234S23619120904
7. Rostom A,Dube C,Wells G,et al. Prevention NSAID-induced gastroduodenal ulcersCochrane Database Syst RevYear: 20024CD00229612519573
8. Shiotani A,Sakakibara T,Yamanaka Y,et al. The preventive factors for aspirin-induced peptic ulcer: aspirin ulcer and corpus atrophyJ GastroenterolYear: 20094471772519448967
9. Silverstein FE,Graham DY,Senior JR,et al. Misoprostol reduces serious gastrointestinal complications in patients with rheumatoid arthritis receiving non-steroidal anti-inflammatory drugs. A randomized, double-blind, placebo-controlled trialAnn Intern MedYear: 19951232412497611589
10. Park SH,Cho CS,Lee OY,et al. Comparison of prevention of NSAID-induced gastrointestinal complications by rebamipide and misoprostol: a randomized, multicenter, controlled trial-STORM STUDYJ Clin Biochem NutrYear: 20074014815518188417
11. Ono S,Kato M,Imai A,et al. Preliminary tial of rebamipide for prevention of low-dose aspirin-inducedgastric injury in healthy subjects: a randomized, double-blind, placebo-controlled, cross-over studyJ Clin Biochem NutrYear: 20094524825319794936
12. Lanas A,Polo-Tomas M,Roncales P,Zapardiel J,Gonzalez M,Santos V. Type of prescription and leves adherence to NSAIDs and gastricprotectors in at-risk GI patientsGastroenterologyYear: 2010138Supple 1S108
13. Arakawa T,Fujiwara Y,Sollano JD,et al. A questionnaire-based survey on the prescription of non-steroidal anti-inflammatory drugs by physicians in East Asian countries in 2007DigestionYear: 20097917718519342858
14. Kawai T,Yamagishi T,Goto S. Circadian variations of gastrointestinal mucosal damage detected with transnasal endoscopy in apparently healthy subjects treated with low-dose aspirin (ASA) for a short periodJ Atheroscler ThrombYear: 20091615516319556719
15. Poulsom R,Begos DE,Modlin IM. Molecular aspects of restitution: functions of trefoil peptidesYale J Biol MedYear: 1996691371469112745
16. Thim L,May FE. Structure of mammalian trefoil factors and functional insightsCell Mol Life SciYear: 2005622956297316374584
17. Hauser F,Poulsom R,Chinery R,et al. hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterusProc Natl Acad Sci USAYear: 199390696169658346203
18. Vestergaard EM,Poulsen SS,Grønbaek H,et al. Development and evaluation of an ELISA for human trefoil factor 3Clin ChemYear: 2002481689169512324485
19. Vestergaard EM,Brynskov J,Ejskjaer K,et al. Immunoassays of human trefoil factors 1 and 2: measured on serum from patients with inflammatory bowel diseaseScand J Clin Lab InvestYear: 20046414615615115253
20. Khan Q,Mehta L. Relevance of platelet-independent effects of aspirin to its salutary effect in atherosclerosis-related eventsJ Atheroscler ThrombYear: 20051218519016141621
21. Konturek JW,Dembiński A,Konturek SJ,Domschke W. Helicobactor pylori and gastric adaptation to repeated aspirin administration in humansJ Physiol PharmacolYear: 1997483833919376621
22. Wong WM,Poulsom R,Whight NA. Trefoil peptideGutYear: 19994489089510323896
23. Hoffmann W,Jagla W. Cell type specific expression of secretary TFF peptides; colocalization with mucins and synthesis in the brainInt Rev CytolYear: 200221314718111837892
24. Rio MC,Bellocq JP,Daniel JY,et al. Brest cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosaScienceYear: 19882417057083041593
25. Tomasetto C,Rio MC,Gautier C,et al. hsp, the domain-duplicated homolog of pS2 protein, is co-expressed with pS2 in stomach but not in brest carcinomaEMBO JYear: 199094074142303034
26. Koitabashi A,Shimada T,Fujii Y,et al. Indometacin up-regulates TFF2 expression in gastric epithelial cellsAliment Pharmacol TherYear: 200420Suppl. 117117615298624
27. Alderman BM,Ulaganathan M,Judd LM,et al. Insights into the mechanisms of gastric adaptation to aspirin-induced injury: a role for regenerating protein but not trefoil peptidesLab InvestYear: 2003831415142514563943
28. McKenzie C,Thim L,Parsons ME. Topical and intravenous administration of trefoil factors protect the gastric mucosa from ethanol-induced injury in the ratAliment Pharmacol TherYear: 2000141033103410930897
29. Suzuki T,Yoshida N,Nakabe N,et al. Prophylactic effect of rebamipide on aspirin-induced gastric lesions and disruption of tight junctional protein zonula occludens-1 distributionJ Pharmacol SciYear: 200810646947718360096
30. Iijima K,Ichikawa T,Okada S,et al. Rebamipide, a cytoprotective drug, increases gastric mucus secretion in human: evaluations with endoscopic gastrin testDig Dis DciYear: 20095415001507
31. De Miguel A,Ibanez B,Badimón JJ. Clinical implications of clopidogrel resistanceThromb HaemostYear: 200810019620318690337
32. Krasopoulos G,Brister SJ,Beattie WS,Buchanan MR. Aspirin ”resistance” and risk of cardiovascular morbidity: systematic review and meta-analysisBMJYear: 20082619519818202034
33. Qiao HL,Hu YR,Tian X,et al. Pharmacokinetics of three proton pump inhibitors in Chinese subjects in relation to the CYP2C19 genotypeEur J Clin PharmacolYear: 20066210711216402242
34. Furuta T,Shirai N,Sugimoto M,Ohashi K,Ishizaki T. Pharmacogenomics of proton pump inhibitorsPharmacogenomicsYear: 2004518120215016609
35. Yamamoto T,Isono A,Mishina Y,et al. Role of gastric mucoprotective drugs: possible effect of rebamipideJ Clin Biochem NutrYear: 201047273120664727
36. Niwa Y,Nakamura M,Ohmiya N,et al. Efficacy of rebamipide for diclofenac-induced small-intestinal mucosal injuries in healthy subjects: a prospective, randomized, double-blinded, placebo-controlled, cross-over studyJ GastroenterolYear: 20084327027618458842
37. Kawai T,Lanas A,Goto S. European physicians don’t like cytoprotective agents?J Clin Biochem NutrYear: 2011496721765610
38. Johnson DA,Cattau EL Jr.,Khan A,Newell DE,Chobanian SJ. Fiberoptic esophagogastroscopy via nasal intubationGastrointest EndoscYear: 19873332333557031
39. Dumortier J,Ponchon T,Scoazec JY,et al. Prospective evaluation of transnasal esophagogastro-duodenoscopy: feasibility and study on performance and toleranceGastrointest EndoscYear: 19994928529110049409
40. Garcia RT,Cello JP,Nguyen MH,et al. Unsedated ultrathin EGD is well accepted when compared with conventional sedated EGD: a multicenter randomized trialGastroenterologyYear: 20031251606161214724812
41. Kawai T,Miyazaki I,Yagi K,et al. Comparison of the effects on cardiopulmonary function of ultrathin transnasal versus normal diameter trans-oral esophagogastro-duodenoscopy in JapanHepatogastroenterologyYear: 20075477077417591059

Article Categories:
  • Original Article

Keywords: Low-dose ASA, transnasal endoscopy, rebamipide, proton pump inhibitor, trefoil factor.

Previous Document:  Relationship between the estimates of desaturase activities and cardiometabolic phenotypes in Korean...
Next Document:  Formation of 7-carboxyheptyl radical induced by singlet oxygen in the reaction mixture of oleic acid...