| The role of survivin and Bcl-2 in zinc-induced apoptosis in prostate cancer cells. | |
| | |
MedLine Citation:
|
PMID: 20822926 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
OBJECTIVES:: To study the effects of zinc treatment on the gene expression levels of survivin and Bcl-2 in prostate cancer cells. MATERIALS AND METHODS:: The effects of zinc exposure on apoptosis were assessed using two human prostate cancer cell lines, LNCaP and PC-3. Zinc-induced apoptosis was measured by Annexin V staining. The direct effect of zinc on the expression levels of zinc transporters (ZnT-1 and ZnT-4) and apoptosis-related genes (Bax, Bcl-2, and survivin) was determined by RT-PCR analysis. RESULTS:: When LNCaP and PC-3 cells were exposed to various concentrations of zinc sulfate for 48 hors, their growth was inhibited in a dose-dependent manner. The levels of zinc in both cell lines treated with zinc sulfate for 24 hours were higher than in untreated cells. Exposure to zinc induced apoptosis and necrosis in LNCaP and PC-3 cells. Apoptosis became more extensive as the treatment time with zinc increased. There was a significant increase in the gene expression levels of ZnT-1 and ZnT-4 in both cell lines treated with zinc sulfate compared with untreated cells. The expression of Bax mRNA was up-regulated, while the expression of Bcl-2 and survivin were decreased in both cell lines following zinc treatment. CONCLUSIONS:: Exposure to zinc sulfate in human prostate cancer cells increased intracellular levels of zinc, which resulted in increased apoptosis. The apoptogenic effect of elevated concentration of zinc could be due either to increased expression of zinc transporters and increased levels of Bax or decreased Bcl-2 and survivin expression. |
| | |
Authors:
|
Ja Hyeon Ku; Soo Yeon Seo; Cheol Kwak; Hyeon Hoe Kim |
Related Documents
:
|
8025206 - Gamma-interferon, interleukin-4 and interleukin-6 in vitro production in old subjects. 10631876 - Immunological changes in the elderly. 19124746 - Ig-like transcript 4 inhibits lipid antigen presentation through direct cd1d interaction. 11739496 - Engagement of ox40 enhances antigen-specific cd4(+) t cell mobilization/memory developm... 1902856 - Abrogation of glucocorticoid-mediated inhibition of t cell proliferation by the synergi... 18444086 - Lack of immune responses against multiple sclerosis-associated retrovirus/human endogen... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2010-9-3 |
Journal Detail:
|
Title: Urologic oncology Volume: - ISSN: 1873-2496 ISO Abbreviation: - Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-9-8 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9805460 Medline TA: Urol Oncol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Urology, Seoul National University Hospital, Seoul, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Survey of current practice related to grading of rejection in cardiac transplant recipients in North...
Next Document: Serum tumor markers in testicular cancer.