Document Detail


The role of subtilisin-like proprotein convertases for cleavage of the measles virus fusion glycoprotein in different cell types.
MedLine Citation:
PMID:  9878618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fusion (F) glycoprotein gene of measles virus (MV) encodes a nonfusogenic precursor protein (F0) that is activated by cleavage into the F1 and F2 subunits during transport to the cell surface. The F protein of both the Edmonston strain and a wild-type MV was found to be cleaved in the trans-Golgi cisternae and/or the trans-Golgi network (TGN). In HEp-2 cells, B lymphoblastoid cells, and PBMC, the cleavage process required calcium, and calcium deprivation prevented syncytium formation. The calcium dependence indicated the involvement of the pro-protein convertase (PC) endoprotease family. The expression of the presently recognized members of the PC family in human cell types known to be infected during measles was examined by RT-PCR. Among the PCs residing in the TGN, only furin was expressed in all cells. Soluble secreted human furin produced by a recombinant baculovirus cleaved MV F0 into proteins the exact size of F1 and F2 and increased the titer of MV particles released from calcium-deprived or endoprotease defective infected cells. These results strongly indicate that furin is the most important and maybe the only endoprotease involved in activation of the MV F protein.
Authors:
G Bolt; I R Pedersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  252     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-01-28     Completed Date:  1999-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  387-98     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Affiliation:
Panum Institute, University of Copenhagen, Blegdamsvej 3, Copenhagen N, 2200, Denmark. G.Bolt@immi.ku.dk
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma
Carcinoma, Squamous Cell
Furin
Golgi Apparatus / enzymology,  virology
Humans
Kinetics
Measles virus / genetics,  physiology*
Plaque Assay
Recombinant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Serine Endopeptidases / genetics,  metabolism*
Subtilisins / metabolism
Tumor Cells, Cultured
Viral Fusion Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 0/Viral Fusion Proteins; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/Subtilisins; EC 3.4.21.75/Furin

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