| The role of sialic acid in the functional activity and the hepatic clearance of C1-INH. | |
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MedLine Citation:
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PMID: 7451969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The immunochemical properties, catabolic clearance, and organ distribution of highly purified radiolabeled human C1-INH were studied after treatment with glycosidases. The antigenicity and C1s inhibitory activity of C1-INH were unimpaired after removal of sialic acid residues (60%) or sialic acid and galactose residues (60 and 20%, respectively) with immobilized neuraminidase and beta-galactosidase. Treatment of C1-INH with neuraminidase was associated with increased cathodal migration of the glycoprotein. In vivo studies in the rabbit, however, showed that removal of sialic acid residues resulted in rapid blood clearance of C1-INH and enhanced localization of the asialo C1-INH in the liver. Subsequent removal of penultimate galactose residues returned the survival time in the circulation and organ distribution to near normal. Additionally, simultaneous infusion of asialo-C1-INH with asialo alpha 1-acid glycoprotein, a protein known to bind to hepatic asialoglycoprotein receptors, dramatically extended the intravascular survival time of asialo C1-INH. In agreement with the observations on other plasma glycoproteins, these results indicate that desialylation of C1-INH results in rapid clearance by hepatic asialoglycoprotein receptors. |
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Authors:
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J O Minta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 126 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1981 Jan |
Date Detail:
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Created Date: 1981-03-27 Completed Date: 1981-03-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 245-9 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Complement C1 Inactivator Proteins / immunology, metabolism* Glycoproteins / pharmacology Humans Kidney / metabolism Liver / metabolism* Male Metabolic Clearance Rate Neuraminidase / pharmacology Rabbits Sialic Acids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Complement C1 Inactivator Proteins; 0/Glycoproteins; 0/Sialic Acids; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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