| The role of serotonin and p53 status in the radiation-induced bystander effect. | |
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MedLine Citation:
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PMID: 22803606 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Purpose: The aim of this study was to investigate the role of serotonin and protein 53 (p53) status of the cells in the radiation-induced bystander effects (RIBE). Materials and methods: The radiation-induced bystander response was investigated in human MCF-7 breast cancer cells and human HCT116 colorectal cancer cells employing medium-transfer experiments and micronuclei (MN) induction as an end-point. Irradiated cell conditioned medium (ICCM) from cells exposed to α-particle or β-radiation was filtered and transferred to unirradiated cells 2 h following irradiation. MCF-7 cells were irradiated with 0.5 Gy α-particles, while HCT116 p53(+/+) and HCT116 p53(-/-) cells were irradiated with 0.5 Gy γ-radiation. Results: Bystander MCF-7 cells, recipient of ICCM from 0.5 Gy α-particle irradiated MCF-7 cells grown in high serotonin conditions showed a modest but significant increase in MN, while MCF-7 cells receiving ICCM with low serotonin levels did not show any bystander effect. Added serotonin (100 ng/ml) led to a bystander effectin HCT116 p53 (-/-) cells recipient of ICCM from 0.5 Gy γ-irradiated HCT116 p53 (+/+) cells, but had no effect when the ICCM was from γ-irradiated HCT116 P53 (-/-) cells. Conclusion: The results indicate that serotonin levels in the medium play a role in the RIBE and that there may be an interaction between the role of serotonin and the p53 status of the irradiated cells. |
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Authors:
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Erta Kalanxhi; Jostein Dahle |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-18 |
Journal Detail:
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Title: International journal of radiation biology Volume: - ISSN: 1362-3095 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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