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The role of serotonin and p53 status in the radiation-induced bystander effect.
MedLine Citation:
PMID:  22803606     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Purpose: The aim of this study was to investigate the role of serotonin and protein 53 (p53) status of the cells in the radiation-induced bystander effects (RIBE). Materials and methods: The radiation-induced bystander response was investigated in human MCF-7 breast cancer cells and human HCT116 colorectal cancer cells employing medium-transfer experiments and micronuclei (MN) induction as an end-point. Irradiated cell conditioned medium (ICCM) from cells exposed to α-particle or β-radiation was filtered and transferred to unirradiated cells 2 h following irradiation. MCF-7 cells were irradiated with 0.5 Gy α-particles, while HCT116 p53(+/+) and HCT116 p53(-/-) cells were irradiated with 0.5 Gy γ-radiation. Results: Bystander MCF-7 cells, recipient of ICCM from 0.5 Gy α-particle irradiated MCF-7 cells grown in high serotonin conditions showed a modest but significant increase in MN, while MCF-7 cells receiving ICCM with low serotonin levels did not show any bystander effect. Added serotonin (100 ng/ml) led to a bystander effectin HCT116 p53 (-/-) cells recipient of ICCM from 0.5 Gy γ-irradiated HCT116 p53 (+/+) cells, but had no effect when the ICCM was from γ-irradiated HCT116 P53 (-/-) cells. Conclusion: The results indicate that serotonin levels in the medium play a role in the RIBE and that there may be an interaction between the role of serotonin and the p53 status of the irradiated cells.
Authors:
Erta Kalanxhi; Jostein Dahle
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-18
Journal Detail:
Title:  International journal of radiation biology     Volume:  -     ISSN:  1362-3095     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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