Document Detail


Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
MedLine Citation:
PMID:  21705547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work investigated the role of rpoS in the development of increased cell envelope resilience and enhanced pressure resistance in stationary-phase cells of Escherichia coli. Loss of both colony-forming ability and membrane integrity, measured as uptake of propidium iodide (PI), occurred at lower pressures in E. coli BW3709 (rpoS) than in the parental strain (BW2952). The rpoS mutant also released much higher concentrations of protein under pressure than the parent. We propose that RpoS-regulated functions are responsible for the increase in membrane resilience as cells enter stationary phase and that this plays a major role in the development of pressure resistance. Strains from the Keio collection with mutations in two RpoS-regulated genes, cfa (cyclopropane fatty acyl phospholipid synthase) and osmB (outer membrane lipoprotein), were significantly more pressure sensitive and took up more PI than the parent strain, with cfa having the greatest effect. Mutations in the bolA morphogene and other RpoS-regulated lipoprotein genes (osmC, osmE, osmY, and ybaY) had no effect on pressure resistance. The cytoplasmic membranes of the rpoS mutant failed to reseal after pressure treatment, and strains with mutations in osmB and nlpI (new lipoprotein) were also somewhat impaired in the ability to reseal their membranes. The cfa mutant, though pressure sensitive, was unaffected in membrane resealing, implying that the initial transient permeabilization event is critical for loss of viability rather than the failure to reseal. The enhanced pressure sensitivity of polA, recA, and xthA mutants suggested that DNA may be a target of oxidative stress in pressure-treated cells.
Authors:
Duangkamol Charoenwong; Simon Andrews; Bernard Mackey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-24
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-29     Completed Date:  2012-01-31     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5220-9     Citation Subset:  IM    
Affiliation:
Department of Food and Nutritional Sciences, The University of Reading, P.O. Box 226, Whiteknights, Reading RG6 6AP, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics*,  metabolism
Base Sequence
Cell Membrane / genetics,  metabolism*
Escherichia coli / genetics,  growth & development,  metabolism*
Escherichia coli Proteins / genetics,  metabolism
Gene Expression Regulation, Bacterial
Mutation
Osmotic Pressure
Pressure
Propidium / metabolism
Sequence Analysis, DNA
Sigma Factor / genetics*,  metabolism
Stress, Physiological / genetics*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Escherichia coli Proteins; 0/Sigma Factor; 0/sigma factor KatF protein, Bacteria; 36015-30-2/Propidium
Comments/Corrections

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