Document Detail


The role of reactive oxygen species in the induction of Ty1 retrotransposition in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  20073031     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here we provide evidence for a dependence between the increased production of reactive oxygen species and the activation of Ty1 retrotransposition. We have found that the strong activator of Ty1 mobility, methylmethane sulphonate, can not induce Ty1 retrotransposition in cells with compromised mitochondrial oxidative phosphorylation (rho(-); sco1Delta), which is the major source for production of reactive oxygen species (ROS) in Saccharomyces cerevisiae. The quantitative estimation of superoxide anions in living cells showed that rho(+) cells exposed to methylmethane sulphonate increase Ty1 retrotransposition and superoxide levels. The increase of superoxide anions by the superoxide generator menadione is accompanied by induction of Ty1 mobility without any treatment with a DNA-damaging agent. Higher frequencies of retrotransposition were found in rho(+) and rho(-) cells treated with exogenously added hydrogen peroxide or in cells with disrupted YAP1 gene characterized by increased intracellular levels of hydrogen peroxide. These data indicate that increased levels of ROS may have an independent and key role in the induction of Ty1 retrotransposition.
Authors:
Teodora Stoycheva; Margarita Pesheva; Pencho Venkov
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  27     ISSN:  1097-0061     ISO Abbreviation:  Yeast     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-21     Completed Date:  2010-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  England    
Other Details:
Languages:  eng     Pagination:  259-67     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 John Wiley & Sons, Ltd.
Affiliation:
Institute of Cryobiology and Food Technology, Department of Cryobiology and Lyophilization, 53A Cherni Vrah Blvd., 1407 Sofia, Bulgaria. teodora.stoycheva@ikht.bg
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MeSH Terms
Descriptor/Qualifier:
Gene Deletion
Hydrogen Peroxide / metabolism,  pharmacology
Methyl Methanesulfonate / pharmacology
Mitochondria / metabolism
Oxidative Phosphorylation
Reactive Oxygen Species / metabolism*
Retroelements*
Saccharomyces cerevisiae / drug effects,  genetics*,  metabolism*
Saccharomyces cerevisiae Proteins / genetics,  metabolism
Superoxides / metabolism
Transcription Factors / genetics,  metabolism
Vitamin K 3 / metabolism
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; 0/Retroelements; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 0/YAP1 protein, S cerevisiae; 11062-77-4/Superoxides; 58-27-5/Vitamin K 3; 66-27-3/Methyl Methanesulfonate; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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