Document Detail

The role of reactive oxygen species and hemeoxygenase-1 expression in the cytotoxicity, cell cycle alteration and apoptosis of dental pulp cells induced by BisGMA.
MedLine Citation:
PMID:  20673999     Owner:  NLM     Status:  MEDLINE    
Biocompatibility of dentin bonding agents (DBAs) and resin composite is important to preserve the pulp vitality after operative restoration. Bisphenol-glycidyl-methacrylate (BisGMA) is one common monomer adding into DBAs and resin. In this study, we found that exposure of human dental pulp cells to BisGMA (>0.1 mM) led to cytotoxicity, G2/M cell cycle arrest and apoptosis as analyzed by propidium iodide (PI) and PI/annexin V dual fluorescent flow cytometry. These events were associated with a decline of cdc2, cdc25C and cyclinB1 expression at both mRNA and protein levels. BisGMA also induced the expression of hemeoxygenase-1 (HO-1), an oxidative stress responsive gene, in pulp cells. Catalase could prevent the BisGMA-induced alteration of cell cycle-related genes (cdc2, cdc25C, cyclinB1) and HO-1 expression in dental pulp cells. Interestingly, Zn-protoporphyrin (2.5-5 microM), a HO inhibitor, enhanced the BisGMA-induced reactive oxygen species (ROS) production and cytotoxicity. These results suggest that exposure to higher concentrations of BisGMA may stimulate ROS production, cell cycle arrest, apoptosis and cell death. Inducing the expression of HO-1 in dental pulp cells by BisGMA is mediated by ROS production and important to protect dental pulp against the toxicity by monomers present in composite resin and DBAs.
Mei-Chi Chang; Lin-I Chen; Chiu-Po Chan; Jang-Jaer Lee; Tong-Mei Wang; Ting-Ting Yang; Po-Shuen Lin; Hsueh-Jen Lin; Hsiao-Hua Chang; Jiiang-Huei Jeng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-31
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  8164-71     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Biomedical Science Team, Chang Gung Institute of Technology, Taoyuan, Taiwan.
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MeSH Terms
Apoptosis / drug effects*
Bisphenol A-Glycidyl Methacrylate / adverse effects*
Catalase / metabolism
Cell Cycle / drug effects*
Cells, Cultured
Dental Pulp / cytology*
Dentin-Bonding Agents / adverse effects*
Gene Expression Regulation / drug effects
Heme Oxygenase-1 / genetics*
Porphyrins / metabolism
Reactive Oxygen Species / metabolism*
Reg. No./Substance:
0/Dentin-Bonding Agents; 0/Porphyrins; 0/Reactive Oxygen Species; 1565-94-2/Bisphenol A-Glycidyl Methacrylate; EC; EC Oxygenase-1

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