Document Detail

The role of proteomics in the assessment of premature rupture of fetal membranes.
MedLine Citation:
PMID:  15970282     Owner:  NLM     Status:  MEDLINE    
The presence and integrity of amniotic fluid is fundamental for the normal development of the human fetus during pregnancy. Its production rate changes throughout pregnancy and is mainly related to the functions of the different fetal, placental and amniotic compartments. Premature rupture of the membranes (PROM) occurs in about 5% of deliveries, with complications such as infection and preterm birth. The management of patients with PROM, regardless of gestational age, remains controversial, and it is therefore important to develop new biological tests in order to achieve accurate diagnoses by identifying the presence of specific amniotic fluid markers in vaginal environment. We recently showed the usefulness of amniotic fluid proteomics in identifying a series of peptides that were absent from the corresponding maternal plasma. Several peptides corresponded to fragments of plasma proteins. Two peptides, absent from plasma samples of pregnant women, were identified in amniotic fluid. They corresponded to the COOH-terminal parts of perlecan (SwissProt: P98160) and of agrin (SwissProt: O00468) protein cores, two major heparan sulfate proteoglycans of basement membranes. In this review we will discuss modern proteomic strategies that may improve the laboratory assessment of PROM, and will focus on some of the biochemical characteristics of agrin and perlecan fragments identified in amniotic fluid.
Lynne Thadikkaran; David Crettaz; Michèle A Siegenthaler; Denis Gallot; Vincent Sapin; Renato V Iozzo; Pierre-Alain Queloz; Philippe Schneider; Jean-Daniel Tissot
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  360     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-05     Completed Date:  2005-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  27-36     Citation Subset:  IM    
Service Régional Vaudois de Transfusion sanguine, Rue du Bugnon 27, CH-1005 Lausanne, Switzerland.
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MeSH Terms
Amino Acid Sequence
Amniotic Fluid / chemistry
Fetal Membranes, Premature Rupture / diagnosis,  etiology*
Heparin / analogs & derivatives,  analysis,  physiology
Molecular Sequence Data
Proteins / analysis
Proteoglycans / analysis,  physiology
Proteomics / methods*
Reg. No./Substance:
0/Proteins; 0/Proteoglycans; 0/heparin proteoglycan; 9005-49-6/Heparin

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