Document Detail


The role of polar phytocomplexes on anticonvulsant effects of leaf extracts of Lippia alba (Mill.) N.E. Brown chemotypes.
MedLine Citation:
PMID:  19589236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The purpose of the present work was to characterize the pharmacological profile of different L. alba chemotypes and to correlate the obtained data to the presence of chemical constituents detected by phytochemical analysis. METHODS: Essential oils from each L. alba chemotype (LP1-LP7) were characterized by gas chromatography-mass spectrometry (GC-MS) and extracted non-volatile compounds were analysed by HPLC and GC-MS. The anticonvulsant actions of the extracted compounds were studied in pentylenetetrazole-induced clonic seizures in mice and their effect on motor coordination was studied using the rota-rod test in rats. The synaptosomes and synaptic membranes of the rats were examined for the influence of LP3 chemotype extract on GABA uptake and binding experiments. KEY FINDINGS: Behavioural parameters encompassed by the pentylenetetrazole test indicated that 80% ethanolic extracts of LP1, LP3 and LP6 L. alba chemotypes were more effective as anticonvulsant agents. Neurochemical assays using synaptosomes and synaptic membranes showed that L. alba LP3 chemotype 80% ethanolic extract inhibited GABA uptake and GABA binding in a dose-dependent manner. HPLC analysis showed that LP1, LP3 and LP6 80% ethanolic extracts presented a similar profile of constituents, differing from those seen in LP2, LP4, LP5 and LP7 80% ethanolic extracts, which exhibited no anticonvulsant effect. GC-MS analysis indicated the occurrence of phenylpropanoids in methanolic fractions obtained from LP1, LP3 and LP6 80% ethanolic extracts and also the accumulation of inositol and flavonoids in hydroalcoholic fractions. CONCLUSIONS: Our results suggest that the anticonvulsant properties shown by L. alba might be correlated to the presence of a complex of non-volatile substances (phenylpropanoids, flavonoids and/or inositols), and also to the volatile terpenoids (beta-myrcene, citral, limonene and carvone), which have been previously validated as anticonvulsants.
Authors:
Antônio C Neto; Joaquim C Netto; Paulo S Pereira; Ana M S Pereira; Sílvia H Taleb-Contini; Suzelei C França; Márcia O M Marques; René O Beleboni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  61     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-10     Completed Date:  2009-08-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  933-9     Citation Subset:  IM    
Affiliation:
Unidade de Biotecnologia, Universidade de Ribeirão Preto, São Paulo, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anticonvulsants / pharmacology,  therapeutic use*
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Flavonoids / pharmacology,  therapeutic use*
Gas Chromatography-Mass Spectrometry
Lippia / chemistry*
Male
Mice
Motor Skills / drug effects
Oils, Volatile / isolation & purification,  pharmacology,  therapeutic use*
Pentylenetetrazole
Phytotherapy
Plant Extracts / chemistry,  pharmacology,  therapeutic use
Plant Leaves / chemistry
Rats
Rats, Wistar
Rotarod Performance Test
Seizures / chemically induced,  drug therapy*
Terpenes / pharmacology,  therapeutic use*
gamma-Aminobutyric Acid / metabolism
Chemical
Reg. No./Substance:
0/Anticonvulsants; 0/Flavonoids; 0/Oils, Volatile; 0/Plant Extracts; 0/Terpenes; 54-95-5/Pentylenetetrazole; 56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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