| The role of polar phytocomplexes on anticonvulsant effects of leaf extracts of Lippia alba (Mill.) N.E. Brown chemotypes. | |
| | |
MedLine Citation:
|
PMID: 19589236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: The purpose of the present work was to characterize the pharmacological profile of different L. alba chemotypes and to correlate the obtained data to the presence of chemical constituents detected by phytochemical analysis. METHODS: Essential oils from each L. alba chemotype (LP1-LP7) were characterized by gas chromatography-mass spectrometry (GC-MS) and extracted non-volatile compounds were analysed by HPLC and GC-MS. The anticonvulsant actions of the extracted compounds were studied in pentylenetetrazole-induced clonic seizures in mice and their effect on motor coordination was studied using the rota-rod test in rats. The synaptosomes and synaptic membranes of the rats were examined for the influence of LP3 chemotype extract on GABA uptake and binding experiments. KEY FINDINGS: Behavioural parameters encompassed by the pentylenetetrazole test indicated that 80% ethanolic extracts of LP1, LP3 and LP6 L. alba chemotypes were more effective as anticonvulsant agents. Neurochemical assays using synaptosomes and synaptic membranes showed that L. alba LP3 chemotype 80% ethanolic extract inhibited GABA uptake and GABA binding in a dose-dependent manner. HPLC analysis showed that LP1, LP3 and LP6 80% ethanolic extracts presented a similar profile of constituents, differing from those seen in LP2, LP4, LP5 and LP7 80% ethanolic extracts, which exhibited no anticonvulsant effect. GC-MS analysis indicated the occurrence of phenylpropanoids in methanolic fractions obtained from LP1, LP3 and LP6 80% ethanolic extracts and also the accumulation of inositol and flavonoids in hydroalcoholic fractions. CONCLUSIONS: Our results suggest that the anticonvulsant properties shown by L. alba might be correlated to the presence of a complex of non-volatile substances (phenylpropanoids, flavonoids and/or inositols), and also to the volatile terpenoids (beta-myrcene, citral, limonene and carvone), which have been previously validated as anticonvulsants. |
| | |
Authors:
|
Antônio C Neto; Joaquim C Netto; Paulo S Pereira; Ana M S Pereira; Sílvia H Taleb-Contini; Suzelei C França; Márcia O M Marques; René O Beleboni |
Related Documents
:
|
11925076 - Pheromone components of the wheat stem sawfly: identification, electrophysiology, and f... 20225896 - Multiresidue pesticide analysis of ginseng powders using acetonitrile- or acetone-based... 19577756 - Programmed temperature vaporizer based method for the sensitive determination of trihal... 12696956 - Investigation of the change in the flavor of a coffee drink during heat processing. 7925246 - A rapid method for detection of n-acetylglucosaminidase-type chitinase activity in cros... 12675356 - Determination of rare-earth elements in a limestone geological standard reference mater... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of pharmacy and pharmacology Volume: 61 ISSN: 0022-3573 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2009 Jul |
Date Detail:
|
Created Date: 2009-07-10 Completed Date: 2009-08-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
|
Languages: eng Pagination: 933-9 Citation Subset: IM |
Affiliation:
|
Unidade de Biotecnologia, Universidade de Ribeirão Preto, São Paulo, Brasil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anticonvulsants / pharmacology, therapeutic use* Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Flavonoids / pharmacology, therapeutic use* Gas Chromatography-Mass Spectrometry Lippia / chemistry* Male Mice Motor Skills / drug effects Oils, Volatile / isolation & purification, pharmacology, therapeutic use* Pentylenetetrazole Phytotherapy Plant Extracts / chemistry, pharmacology, therapeutic use Plant Leaves / chemistry Rats Rats, Wistar Rotarod Performance Test Seizures / chemically induced, drug therapy* Terpenes / pharmacology, therapeutic use* gamma-Aminobutyric Acid / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Anticonvulsants; 0/Flavonoids; 0/Oils, Volatile; 0/Plant Extracts; 0/Terpenes; 54-95-5/Pentylenetetrazole; 56-12-2/gamma-Aminobutyric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protection afforded by a herbal medicine, Sho-seiryu-to (TJ-19), against oleic acid-induced acute lu...
Next Document: Protection of DNA and membranes from gamma-radiation induced damages by Centella asiatica.