| A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance. | |
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MedLine Citation:
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PMID: 18776900 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation. |
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Authors:
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Jia-Shu Yang; Helge Gad; Stella Y Lee; Alexander Mironov; Leiliang Zhang; Galina V Beznoussenko; Carmen Valente; Gabriele Turacchio; Akua N Bonsra; Guangwei Du; Gianluca Baldanzi; Andrea Graziani; Sylvain Bourgoin; Michael A Frohman; Alberto Luini; Victor W Hsu |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-09-07 |
Journal Detail:
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Title: Nature cell biology Volume: 10 ISSN: 1476-4679 ISO Abbreviation: Nat. Cell Biol. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-02 Completed Date: 2008-10-27 Revised Date: 2011-11-24 |
Medline Journal Info:
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Nlm Unique ID: 100890575 Medline TA: Nat Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 1146-53 Citation Subset: IM |
Affiliation:
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Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals COP-Coated Vesicles / enzymology, metabolism*, ultrastructure COS Cells Carrier Proteins / chemistry, metabolism Cercopithecus aethiops Golgi Apparatus / enzymology, metabolism*, ultrastructure HeLa Cells Humans Intracellular Membranes / metabolism Liposomes / metabolism Mice Phosphatidic Acids / metabolism* Phospholipase D / metabolism Protein Structure, Tertiary |
| Grant Support | |
ID/Acronym/Agency:
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GGP04235//Telethon; GM058615/GM/NIGMS NIH HHS; GM071475/GM/NIGMS NIH HHS; GM071520/GM/NIGMS NIH HHS; R01 GM058615-07/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Liposomes; 0/Phosphatidic Acids; EC 3.1.4.-/phospholipase D2; EC 3.1.4.4/Phospholipase D |
| Comments/Corrections | |
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