Document Detail

A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance.
MedLine Citation:
PMID:  18776900     Owner:  NLM     Status:  MEDLINE    
Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.
Jia-Shu Yang; Helge Gad; Stella Y Lee; Alexander Mironov; Leiliang Zhang; Galina V Beznoussenko; Carmen Valente; Gabriele Turacchio; Akua N Bonsra; Guangwei Du; Gianluca Baldanzi; Andrea Graziani; Sylvain Bourgoin; Michael A Frohman; Alberto Luini; Victor W Hsu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-09-07
Journal Detail:
Title:  Nature cell biology     Volume:  10     ISSN:  1476-4679     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-02     Completed Date:  2008-10-27     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1146-53     Citation Subset:  IM    
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MeSH Terms
COP-Coated Vesicles / enzymology,  metabolism*,  ultrastructure
COS Cells
Carrier Proteins / chemistry,  metabolism
Cercopithecus aethiops
Golgi Apparatus / enzymology,  metabolism*,  ultrastructure
HeLa Cells
Intracellular Membranes / metabolism
Liposomes / metabolism
Phosphatidic Acids / metabolism*
Phospholipase D / metabolism
Protein Structure, Tertiary
Grant Support
GGP04235//Telethon; GM058615/GM/NIGMS NIH HHS; GM071475/GM/NIGMS NIH HHS; GM071520/GM/NIGMS NIH HHS; R01 GM058615/GM/NIGMS NIH HHS; R01 GM058615-07/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Carrier Proteins; 0/Liposomes; 0/Phosphatidic Acids; EC 3.1.4.-/phospholipase D2; EC D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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