Document Detail

The role of p27 in controlling the oxygen-dependent checkpoint of mammalian cells in late G1.
MedLine Citation:
PMID:  16158973     Owner:  NLM     Status:  MEDLINE    
We have studied hypoxia-induced cell cycle arrest in human cells where the retinoblastoma tumour suppressor protein (pRb) is either functional (T-47D cells) or abrogated by expression of the HPV18 E7 oncoprotein (NHIK 3025 cells). Cells of both types are arrested in a restriction point in late G1, here denoted as the oxygen-dependent restriction point in late G1. This arrest seems to occur under extreme hypoxia in all types of mammalian cells so far tested. During an 18-h exposure to extreme hypoxia, the p27 protein level increased in G1-phase in both cells lines investigated and was followed by a binding between p27 and CDK2. This was observed both in the pRb-positive T-47D cells and in the pRb-negative NHIK 3025 cells. We, therefore, believe that p27 and not pRb is the mediator of this oxygen-dependent checkpoint in late G1. Our results also suggest that p27 regulates the restart of cell cycle progression of these arrested cells after reoxygenation.
Pål Graff; Oystein Amellem; Jo Seim; Trond Stokke; Erik O Pettersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  25     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-09-14     Completed Date:  2005-11-02     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2259-67     Citation Subset:  IM    
Department of Physics, The Biophysics Group, University of Oslo, P.O. Box 1048 Blindern, 0316 Oslo, Norway.
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MeSH Terms
Breast Neoplasms / metabolism,  pathology*
CDC2-CDC28 Kinases / metabolism*
Cell Cycle / physiology
Cell Cycle Proteins / metabolism*
Cell Hypoxia / physiology
Cell Line, Tumor
Cyclin E / metabolism
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinase Inhibitor p27
G1 Phase / physiology*
Oxygen / metabolism,  pharmacology
Tumor Suppressor Proteins / metabolism*
Uterine Cervical Neoplasms / metabolism,  pathology*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Cyclin E; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 7782-44-7/Oxygen; EC Kinases; EC protein, human; EC Kinase 2

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