| The role of molecular analysis of immunoglobulin and T cell receptor gene rearrangements in the diagnosis of lymphoproliferative disorders. | |
| | |
MedLine Citation:
|
PMID: 11429433 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIMS: To investigate whether the analysis of immunoglobulin (Ig)/T cell receptor (TCR) rearrangements is useful in the diagnosis of lymphoproliferative disorders. METHODS: In a series of 107 consecutive cases with initial suspicion of non-Hodgkin's lymphoma (NHL), Southern blot (SB) analysis of Ig/TCR rearrangements was performed. RESULTS: In 98 of 100 histopathologically conclusive cases, Ig/TCR gene results were concordant. In one presumed diffuse large B cell lymphoma (DLCL) and one follicular lymphoma (FL) case no clonality could be detected by SB analysis, or by polymerase chain reaction (PCR) at second stage. In the DLCL, sampling error might have occurred; the FL was revised after an initial diagnosis of reactivity. In many of the histopathologically inconclusive cases Ig/TCR gene SB analysis was helpful, giving support for the histopathological suspicion. However, because of a lack of (clinical) follow up data this could not be confirmed in a few cases. CONCLUSIONS: Experienced haematopathologists or a pathologist panel can diagnose malignant versus reactive lesions in most cases without the need for Ig/TCR gene analysis and can select the 5-10% of cases that might benefit from molecular clonality studies. |
| | |
Authors:
|
A W Langerak; J H van Krieken; I L Wolvers-Tettero; E Kerkhof; A H Mulder; L W Vrints; J W Coebergh; E Schuuring; P M Kluin; J J van Dongen |
Related Documents
:
|
21750133 - Retroperitoneal oncocytoma: case report and review of the imaging features. 12867893 - Dynamic smile visualization and quantification: part 1. evolution of the concept and dy... 21617553 - Rapid development of sarcoid tenosynovitis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of clinical pathology Volume: 54 ISSN: 0021-9746 ISO Abbreviation: J. Clin. Pathol. Publication Date: 2001 Jul |
Date Detail:
|
Created Date: 2001-06-28 Completed Date: 2001-08-02 Revised Date: 2008-11-20 |
Medline Journal Info:
|
Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: England |
Other Details:
|
Languages: eng Pagination: 565-7 Citation Subset: AIM; IM |
Affiliation:
|
Department of Immunology, University Hospital Rotterdam/Erasmus University Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands. langerak@immu.fgg.eur.nl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blotting, Southern Diagnosis, Differential Follow-Up Studies Gene Rearrangement, T-Lymphocyte / genetics* Genes, Immunoglobulin* Humans Lymphoma, Non-Hodgkin / diagnosis*, pathology Lymphoproliferative Disorders / diagnosis Prospective Studies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lymphadenopathy and lymph node infarction as a result of gold injections.
Next Document: Predominant fatty variant of myofibroblastoma of breast.