| The role of mitochondrial complex III in melatonin-induced ROS production in cultured mesangial cells. | |
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MedLine Citation:
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PMID: 20969621 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Melatonin is a potent scavenger of reactive oxygen (ROS) and reactive nitrogen species (RNS). At pharmacological concentrations, however, melatonin is documented to cause ROS/RNS production, especially in cultured cancerous cells. Currently, the mechanism responsible for melatonin-induced ROS generation remains elusive. In this study, we provided evidence that melatonin, at micromolar concentrations, induced rapid ROS generation by a mitochondrial-dependent mechanism in primary human mesangial (HM) cells. The melatonin-induced ROS production occurred independent of changes in Ca(2+) concentrations in the cytosol and/or in mitochondria. In mitochondria isolated from HM cells and mice kidney tissues, melatonin caused ROS production; this melatonin response was completely blocked by the complex III inhibitor antimycin A. In contrast, both the mitochondrial complex I inhibitor, rotenone, and another complex III inhibitor, myxothiazol, which interacts with complex III at a distinct site, had no significant inhibitory effect on melatonin-induced ROS generation. These results demonstrate that melatonin induced rapid ROS generation via the antimycin A-sensitive site of mitochondrial complex III. |
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Authors:
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Hong-Mei Zhang; Yiqiang Zhang; Bin-Xian Zhang |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-20 |
Journal Detail:
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Title: Journal of pineal research Volume: 50 ISSN: 1600-079X ISO Abbreviation: J. Pineal Res. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-14 Completed Date: 2011-04-05 Revised Date: 2012-01-04 |
Medline Journal Info:
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Nlm Unique ID: 8504412 Medline TA: J Pineal Res Country: Denmark |
Other Details:
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Languages: eng Pagination: 78-82 Citation Subset: IM |
Copyright Information:
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Journal of Pineal Research © 2010 John Wiley & Sons A/S. No claims to US government works. |
Affiliation:
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Departments of MedicinePhysiology, University of Texas Health Science Center at San Antonio, Geriatric Research, Education and Clinical Center, South Texas Veterans Health Care System, Audie L Murphy Division, San Antonio, TX 78229, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Cells, Cultured Electron Transport Complex III / metabolism* Humans Melatonin / pharmacology* Mesangial Cells / drug effects*, metabolism* Mice Mitochondria / drug effects, metabolism Reactive Nitrogen Species / metabolism Reactive Oxygen Species / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HL075011/HL/NHLBI NIH HHS; R01 HL075011-04/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 73-31-4/Melatonin; 7440-70-2/Calcium; EC 1.10.2.2/Electron Transport Complex III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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