| A role for the lysosomal protease cathepsin B in zebrafish follicular apoptosis. | |
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MedLine Citation:
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PMID: 20170740 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study presents evidence that cathepsin B, a lysosomal protease, may be involved in the regulation of apoptosis during serum-starvation in teleost follicles. Zebrafish vitellogenic follicles were isolated, incubated under serum-free conditions and homogenized. The follicle extracts demonstrated caspase-3-like activity using the fluorogenic substrate DEVD-AMC, indicating the onset of apoptosis. Cathepsin B activity as measured using the fluorogenic cathepsin B substrate, Z-Arg-Arg-AMC was elevated within the first 6h of incubation in serum-free media and coincided with the onset of apoptosis. This increase in cathepsin B activity was sensitive to the cathepsin B inhibitor, CA-074-ME. Furthermore, adding CA-074-ME to the follicle incubation blocked caspase-3-like activation, suggesting that cathepsin B activity is a positive regulator of the apoptotic cascade during serum-starvation. Interestingly, the increase in cathepsin-B-like activity was not preceded by an increase in cathepsin B mRNA transcription, suggesting that regulation of this enzyme is at a level other than of the gene. These results suggest a regulatory role for cathepsin B during follicular apoptosis in zebrafish ovarian follicles. |
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Authors:
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Angela J Eykelbosh; Glen Van Der Kraak |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-16 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Volume: 156 ISSN: 1531-4332 ISO Abbreviation: Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-05 Completed Date: 2010-06-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806096 Medline TA: Comp Biochem Physiol A Mol Integr Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 218-23 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* / drug effects Caspase 3 / metabolism Cathepsin B / antagonists & inhibitors*, genetics, metabolism Cell Survival / drug effects Chorionic Gonadotropin / pharmacology Cloning, Molecular Culture Media, Serum-Free Dipeptides / pharmacology Estradiol / pharmacology Female Gene Expression Regulation, Enzymologic / drug effects Humans Lysosomes / drug effects, enzymology* Ovarian Follicle / cytology*, drug effects, enzymology* RNA, Messenger / genetics, metabolism Zebrafish / metabolism* Zebrafish Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/CA 074 methyl ester; 0/Chorionic Gonadotropin; 0/Culture Media, Serum-Free; 0/Dipeptides; 0/RNA, Messenger; 0/Zebrafish Proteins; 50-28-2/Estradiol; EC 3.4.22.-/Caspase 3; EC 3.4.22.1/Cathepsin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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