| The role of lipoprotein lipase in the metabolism of triglyceride-rich lipoproteins by macrophages. | |
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MedLine Citation:
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PMID: 6874679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that cultured macrophages secrete lipoprotein lipase (LPL) into the culture medium. The purpose of these experiments was to determine the role of LPL in the uptake of very low density lipoproteins (VLDL). Both J774 cells and mouse peritoneal macrophages took up and degraded normotriglyceridemic VLDL in a saturable manner. Uptake of VLDL was effectively competed for by rabbit beta-VLDL, human VLDL, but not by native LDL or acetyl LDL. LPL activity accelerated saturable uptake of VLDL but was not a prerequisite for it. This was most clearly seen in experiments with apo-C-II-deficient VLDL. Although no hydrolysis of VLDL triglyceride occurred, saturable uptake and degradation of the C-II-deficient lipoprotein occurred. However, addition of apo-C-II enhanced saturable uptake. Normal VLDL also promoted cellular accumulation of both triglycerides and cholesteryl esters. Accumulation of triglyceride occurred by uptake of intact VLDL particles, by uptake of a triglyceride-depleted particle produced by the action of LPL, and by the direct uptake of free fatty acids generated by the activity of LPL. In turn, the latter process was influenced by the amount of albumin in the medium capable of being an acceptor for free fatty acids. Finally, we have shown that when albumin is present in the medium, the macrophage is capable of mobilizing most of its stored triglyceride over 24 h, even as its cholesteryl ester content remains constant. These studies may be relevant to the ability of VLDL to promote macrophage accumulation of cholesteryl ester, even as triglyceride accumulation is minimized. |
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Authors:
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P Lindqvist; A M Ostlund-Lindqvist; J L Witztum; D Steinberg; J A Little |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 258 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1983 Aug |
Date Detail:
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Created Date: 1983-09-09 Completed Date: 1983-09-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9086-92 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoprotein C-II Apolipoproteins / pharmacology Apolipoproteins C* Chloroquine / pharmacology Cholesterol / analysis Fatty Acids, Nonesterified / pharmacology Female Guinea Pigs Humans Lipids / analysis Lipoprotein Lipase / metabolism* Lipoproteins, LDL / metabolism Lipoproteins, VLDL / metabolism* Macrophages / metabolism* Mice |
| Grant Support | |
ID/Acronym/Agency:
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HL-14197/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein C-II; 0/Apolipoproteins; 0/Apolipoproteins C; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 54-05-7/Chloroquine; 57-88-5/Cholesterol; EC 3.1.1.34/Lipoprotein Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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