| The role of intracellular zinc in modulation of life and death of Hep-2 cells. | |
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MedLine Citation:
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PMID: 12572688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Varying intracellular concentrations of zinc in laryngeal Hep-2 cells in relation to changing cultivation conditions in vitro were determined by atomic absorption spectrophotometry. Upon standard cultivation in DMEM with 10% serum, the mean concentration of zinc was determined at 0.88 +/- 0.09 microg/mg protein, with substantially decreased values in the cells exposed to a low-serum medium. Next, the study of the effects of a series of physiological and supraphysiological concentrations of ZnSO4 on laryngeal cells and their correlation with determined intracellular concentrations of zinc was performed. It was found that zinc concentrations above 100 microM were toxic to Hep-2 cells, inducing cell death in the interval of 96 h as determined by videomicroscopy, selective nuclear staining, and immunofluorescence detection of caspase-3 and specific cytokeratin 18 fragment. Both types of cell death were observed, with apoptosis being induced at moderately toxic zinc concentration of 150 microM and necrosis at higher zinc concentrations of 300 microM and 750 microM, respectively. Lower concentrations (1.5-100 microM), on the other hand, did not produce any measurable changes in cell morphology and function in the same time interval. Zinc at concentration of 1.5 microM was found to slightly enhance proliferation of Hep-2 cells up to the certain time point, which seemed to correlate with maximal tolerable momentary intracellular level of zinc. These results illustrate the importance of determining the intracellular levels of zinc when trying to characterize the effect of exogenous zinc on life and death of laryngeal cells. |
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Authors:
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Emil Rudolf; Kamil Rudolf; Jakub Radocha; Jan Peychl; Miroslav Cervinka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine Volume: 16 ISSN: 0966-0844 ISO Abbreviation: Biometals Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-02-07 Completed Date: 2003-12-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9208478 Medline TA: Biometals Country: Netherlands |
Other Details:
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Languages: eng Pagination: 295-309 Citation Subset: IM |
Affiliation:
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Department of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine in Hradec Králové, Simkova 870, Hradec Králové 1, 500 01, Czech Republic. rudolf@lfhk.cuni.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Cell Line, Tumor Cell Nucleus / pathology Cell Size / drug effects Cell Survival / drug effects Dose-Response Relationship, Drug Humans Indoles Intracellular Fluid / chemistry, metabolism* Necrosis Zinc / analysis, metabolism*, pharmacology*, toxicity Zinc Sulfate / analysis, metabolism, pharmacology, toxicity |
| Chemical | |
Reg. No./Substance:
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0/Indoles; 47165-04-8/DAPI; 7440-66-6/Zinc; 7733-02-0/Zinc Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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