Document Detail


The role of interleukin-6 in the formation of the coronary vasculature.
MedLine Citation:
PMID:  19709461     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The formation and the patterning of the coronary vasculature are critical to the development and pathology of the heart. Alterations in cytokine signaling and biomechanical load can alter the vascular distribution of the vessels within the heart. Changes in the physical patterning of the vasculature can have significant impacts on the relationships of the pressure-flow network and distribution of critical growth and survival factors to the tissue. Interleukin-6 (IL-6) is a pleiotropic cytokine that regulates several biological processes, including vasculogenesis. Using both immunohistological and cardioangiographic analyses, we tested the hypothesis that IL-6-loss will result in decreased vessel density, along with changes in vascular distribution. Moreover, given the impact of vascular patterning on pressure-flow and distribution mechanics, we utilized non-Euclidean geometrical fractal analysis to quantify the changes in patterning resulting from IL-6-loss. Our analyses revealed that IL-6-loss results in a decreased capillary density and increase in intercapillary distances, but does not alter vessel size or diameter. We also observed that the IL-6-/- coronary vasculature had a marked increase in fractal dimension (D value), indicating that IL-6-loss alters vascular patterning. Characterization of IL-6-loss on coronary vasculature may lend insight into the role of IL-6 in the formation and patterning of the vascular bed.
Authors:
Indroneal Banerjee; John W Fuseler; Colby A Souders; Stephanie L K Bowers; Troy A Baudino
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-08-27
Journal Detail:
Title:  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada     Volume:  15     ISSN:  1435-8115     ISO Abbreviation:  Microsc. Microanal.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-16     Completed Date:  2009-11-06     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  9712707     Medline TA:  Microsc Microanal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  415-21     Citation Subset:  IM    
Affiliation:
Cell and Developmental Biology and Anatomy, University of South Carolina School of Medicine, 6439 Garners Ferry Rd., Columbia, SC 29209, USA.
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MeSH Terms
Descriptor/Qualifier:
Angiography
Animals
Coronary Vessels / growth & development*
Immunohistochemistry
Interleukin-6 / deficiency,  physiology*
Mice
Mice, Inbred C57BL
Mice, Knockout
Neovascularization, Physiologic*
Grant Support
ID/Acronym/Agency:
1R01 HL85847/HL/NHLBI NIH HHS; R01 HL085847-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Interleukin-6
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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