| The role of intercellular contacts in the activation of B lymphocytes by anti-immunoglobulin antibodies. | |
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MedLine Citation:
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PMID: 3872324 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been proposed that anti-Ig antibody activates B cells in a way analogous to the antigens, i.e., it delivers its signal by cross-linking and clustering the Ig receptors on the surface of one cell. However, the cross-linking of different B cells which may deliver to each other a signal has not been considered. Thus, we examined the effect of preventing cell contacts on the response of rabbit B cells to anti-Ig allotype antibody by using a solid agarose medium. First, we examined the 14C-uridine incorporation in liquid medium and in liquid or solid agarose in cultures stimulated with anti-Ig antibody (Ab). The response was high in liquid agarose or liquid medium but was absent when the agarose-containing medium was solidified at the start of the cultures. If the agarose was solidified 6 hr after the start, a good response was obtained. Moreover, if the cells were sedimented at the start before solidifying the agarose-containing medium, a good response was also obtained. Similar results were obtained when T cells were activated by Con A. To examine whether B cells require contacts with other B cells or with non-B cells, we examined their response to anti-Ig Ab in the absence of macrophages or T cells, and found that purified B cells from lymphoid organs or from thoracic duct responded well in the absence of T cells and/or macrophages. The response was also absent when the cells were cultured in agar at a "local" concentration close to 10(8) cell/ml. Also, concentrated supernatants of anti-Ig-stimulated cells did not increase the response to anti-Ig in solid agarose. These two last observations suggest that the lack of response in solid agarose is not due to a lack of diffusible factors or to a lack of feeder cells. Therefore, because the only difference between the cultures that responded to anti-Ig or Con A and those that did not was the distance between the cells, we concluded that the contact between B cells or between T cells is essential to their activation by their respective mitogens. We speculate that the anti-Ig Ab or the antigen cross-links B cells, which then provide each other with the activating signal or with one of the activating signals. |
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Authors:
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H Spieker-Polet; K Hagen; M Teodorescu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 134 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1985 May |
Date Detail:
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Created Date: 1985-05-23 Completed Date: 1985-05-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2827-34 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Anti-Idiotypic* / physiology B-Lymphocytes / immunology* Cell Communication* / drug effects Cell Count Concanavalin A / pharmacology Culture Media Interleukin-2 / physiology Lymphocyte Activation* / drug effects Rabbits Receptors, Antigen, B-Cell / immunology Sepharose / pharmacology Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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21399//PHS HHS; AM 28469/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Anti-Idiotypic; 0/Culture Media; 0/Interleukin-2; 0/Receptors, Antigen, B-Cell; 11028-71-0/Concanavalin A; 9012-36-6/Sepharose |
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