Document Detail


The role of IGFs in the regulation of ovarian follicular growth in the brushtail possum (Trichosurus vulpecula).
MedLine Citation:
PMID:  20522481     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IGFs are known to be key regulators of ovarian follicular growth in eutherian mammals, but little is known regarding their role in marsupials. To better understand the potential role of IGFs in the regulation of follicular growth in marsupials, expression of mRNAs encoding IGF1, IGF2, IGF1R, IGF-binding protein 2 (IGFBP2), IGFBP4 and IGFBP5 was localized by in situ hybridization in developing ovarian follicles of the brushtail possum. In addition, the effects of IGF1 and IGF2 on granulosa cell function were tested in vitro. Both granulosa and theca cells synthesize IGF mRNAs, with the theca expressing IGF1 mRNA and granulosa cell expressing IGF2 mRNA. Oocytes and granulosa cells express IGF1R. Granulosa and theca cells expressed IGFBP mRNAs, although the pattern of expression differed between the BPs. IGFBP5 mRNA was differentially expressed as the follicles developed with granulosa cells of antral follicles no longer expressing IGFBP5 mRNA, suggesting an increased IGF bioavailability in the antral follicle. The IGFBP protease, PAPPA mRNA, was also expressed in granulosa cells of growing follicles. Both IGF1 and IGF2 stimulated thymidine incorporation but had no effect on progesterone production. Thus, IGF may be an important regulator of ovarian follicular development in marsupials as has been shown in eutherian mammals.
Authors:
Jennifer L Juengel; Lisa J Haydon; Brigitta Mester; Brian P Thomson; Michael Beaumont; Douglas C Eckery
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-03
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  140     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2010-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  295-303     Citation Subset:  IM    
Affiliation:
Wallaceville Animal Research Centre, AgResearch, Upper Hutt 5140, New Zealand AgResearch Ltd, Invermay Agricultural Centre, Puddle Alley, Mosgiel 9053, New Zealand. jenny.juengel@agresearch.co.nz
Data Bank Information
Bank Name/Acc. No.:
GENBANK/GU250879;  GU250880;  GU250881;  GU250882;  GU250883;  GU250884;  GU250885
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Female
Granulosa Cells / cytology,  physiology*
In Situ Hybridization / veterinary
Insulin-Like Growth Factor Binding Proteins / genetics,  physiology
Insulin-Like Growth Factor I / genetics,  physiology*
Insulin-Like Growth Factor II / genetics,  physiology*
Molecular Sequence Data
Ovarian Follicle / cytology,  growth & development,  physiology*
Pregnancy-Associated Plasma Protein-A / genetics,  physiology
RNA, Messenger / chemistry,  genetics
Receptor, IGF Type 1 / genetics,  physiology
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Analysis, DNA
Trichosurus / physiology*
Chemical
Reg. No./Substance:
0/Insulin-Like Growth Factor Binding Proteins; 0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II; EC 2.7.10.1/Receptor, IGF Type 1; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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