| A role for PPARα in the regulation of arginine metabolism and nitric oxide synthesis. | |
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MedLine Citation:
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PMID: 21063737 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The pleiotropic effects of PPARα may include the regulation of amino acid metabolism. Nitric oxide (NO) is a key player in vascular homeostasis. NO synthesis may be jeopardized by a differential channeling of arginine toward urea (via arginase) versus NO (via NO synthase, NOS). This was studied in wild-type (WT) and PPARα-null (KO) mice fed diets containing either saturated fatty acids (COCO diet) or 18:3 n-3 (LIN diet). Metabolic markers of arginine metabolism were assayed in urine and plasma. mRNA levels of arginases and NOS were determined in liver. Whole-body NO synthesis and the conversion of systemic arginine into urea were assessed by using (15)N(2)-guanido-arginine and measuring urinary (15)NO(3) and [(15)N]-urea. PPARα deficiency resulted in a markedly lower whole-body NO synthesis, whereas the conversion of systemic arginine into urea remained unaffected. PPARα deficiency also increased plasma arginine and decreased citrulline concentration in plasma. These changes could not be ascribed to a direct effect on hepatic target genes, since NOS mRNA levels were unaffected, and arginase mRNA levels decreased in KO mice. Despite the low level in the diet, the nature of the fatty acids modulated some effects of PPARα deficiency, including plasma arginine and urea, which increased more in KO mice fed the LIN diet than in those fed the COCO diet. In conclusion, PPARα is largely involved in normal whole-body NO synthesis. This warrants further study on the potential of PPARα activation to maintain NO synthesis in the initiation of the metabolic syndrome. |
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Authors:
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Najoua Guelzim; François Mariotti; Pascal G P Martin; Frédéric Lasserre; Thierry Pineau; Dominique Hermier |
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Publication Detail:
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Type: Journal Article Date: 2010-11-10 |
Journal Detail:
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Title: Amino acids Volume: 41 ISSN: 1438-2199 ISO Abbreviation: Amino Acids Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: Austria |
Other Details:
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Languages: eng Pagination: 969-79 Citation Subset: IM |
Affiliation:
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UMR914 Nutrition Physiology and Ingestive Behavior, INRA, 16 rue Claude Bernard, 75005, Paris, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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