| The role of C/EBPdelta in the early stages of adipogenesis. | |
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MedLine Citation:
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PMID: 19233245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adipocyte differentiation is a complex process triggered and facilitated by transcription factors such as peroxisome proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein (C/EBP) alpha. Most about the cascade underlying the differentiation process, especially events in the early stages, remain to be elucidated. Early on in adipocyte differentiation, the C/EBPbeta and C/EBPdelta genes are rapidly induced to express and later activate PPARgamma and C/EBPalpha expression. C/EBPbeta also plays a crucial role in mitotic clonal expansion (MCE), the approximately two rounds of mitosis which occurs soon after preadipocytes are stimulated by differentiation inducers and a necessary step for adipocyte differentiation. However, the effect of C/EBPdelta, another member of the C/EBP family, on MCE remains unclear. In the present study, we investigated the role of C/EBPdelta in the early stages of adipogenesis. A remarkable induction of C/EBPdelta gene expression after the initiation of differentiation was observed not in proliferating preadipocytes, but in growth-arrested, differentiable cells. RNAi-mediated knockdown of C/EBPdelta dramatically suppressed cell growth after differentiation was induced, and inhibited conversion into lipid-laden adipocytes. Furthermore, silencing of C/EBPdelta impaired the expression of factor for adipocyte differentiation (fad) 49, which is up-regulated and plays a crucial role early in adipogenesis. Taken together, these findings show that C/EBPdelta is involved in MCE and gene expression in the early stages of adipocyte differentiation. |
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Authors:
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Tomoaki Hishida; Makoto Nishizuka; Shigehiro Osada; Masayoshi Imagawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-20 |
Journal Detail:
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Title: Biochimie Volume: 91 ISSN: 1638-6183 ISO Abbreviation: Biochimie Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-14 Completed Date: 2009-06-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 654-7 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells Adipogenesis / genetics, physiology* Animals CCAAT-Enhancer-Binding Protein-delta / genetics, physiology* Cell Differentiation / genetics, physiology* Gene Expression Regulation, Developmental* / genetics, physiology Mice Mitosis / genetics, physiology Phospholipid Transfer Proteins / genetics, physiology RNA Interference Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Fad49 protein, mouse; 0/Phospholipid Transfer Proteins; 142662-43-9/CCAAT-Enhancer-Binding Protein-delta |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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