| The role of hepatocyte growth factor/c-Met in chronic rhinosinusitis with nasal polyps. | |
| | |
MedLine Citation:
|
PMID: 20819464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Hepatocyte growth factor (HGF) is a growth factor thought to attenuate Th2-driven eosinophilic airway inflammatory responses. Increased expression of HGF and its receptor c-Met in nasal polyps suggests a role in disease pathogenesis. The effect of HGF on human sinonasal epithelial cell (SNEC) responses to Th2 inflammatory cytokines in chronic rhinosinusitis with nasal polyps (CRSwNP) has not been explored. METHODS: SNECs isolated from patients with CRSwNP and control subjects were grown in cell culture at the air-liquid interface. The Th2 cytokine IL-13 was applied for 24 hours in the presence or absence of HGF. Eotaxin-3 and c-Met expression was assessed using real-time PCR, immunohistochemistry, and flow cytometry. RESULTS: SNECs obtained from both CRSwNP and control subjects showed markedly increased expression of eotaxin-3 after exposure to IL-13. HGF significantly blocked IL-13-induced expression of eotaxin-3 in control SNECs, but not in SNECs derived from CRSwNP subjects. CONCLUSION: SNECs are active participants in sinonasal mucosal immunity, expressing inflammatory mediators in response to potential pathogens and endogenous cytokines. Although Th2 cytokines can elicit expression of proeosinophilic mediators by SNECs, HGF appears to have a down-regulating effect on this response. In patients with CRSwNP, SNECs are resistant to this attenuation, showing continued IL-13-induced eotaxin-3 expression despite HGF treatment. Abnormalities in the regulation of epithelial cell responses to endogenous cytokines and growth factors may contribute to the persistent eosinophilic inflammatory state in CRSwNP. |
| | |
Authors:
|
Douglas D Reh; Murugappan Ramanathan; Babar Sultan; Yadong Wang; Lindsey May; Andrew P Lane |
Related Documents
:
|
20421464 - Peroxisome proliferator-activated receptor gamma activation is required for maintenance... 19540934 - Ppar gamma protects cardiomyocytes against oxidative stress and apoptosis via bcl-2 upr... 17579114 - Effects of thiazolidinediones on differentiation, proliferation, and apoptosis. 16303334 - Inflammatory modulation of ppar gamma expression and activity. 1643174 - Immunohistochemical observations on pneumonic lesions caused by rhodococcus equi in foals. 18506634 - Inhibitory effect of pacap on caspase activity in neuronal apoptosis: a better understa... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: American journal of rhinology & allergy Volume: 24 ISSN: 1945-8932 ISO Abbreviation: Am J Rhinol Allergy Publication Date: 2010 Jul-Aug |
Date Detail:
|
Created Date: 2010-09-07 Completed Date: 2011-03-15 Revised Date: 2011-11-10 |
Medline Journal Info:
|
Nlm Unique ID: 101490775 Medline TA: Am J Rhinol Allergy Country: United States |
Other Details:
|
Languages: eng Pagination: 266-70 Citation Subset: IM |
Affiliation:
|
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-0910, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Separation Cells, Cultured Chemokines, CC / biosynthesis, genetics Chronic Disease Disease Progression Epithelial Cells / immunology, metabolism*, pathology Flow Cytometry Hepatocyte Growth Factor / immunology, metabolism* Humans Interleukin-13 / immunology, metabolism Nasal Polyps Paranasal Sinuses / pathology* Rhinitis / immunology*, pathology, physiopathology Sinusitis / immunology*, pathology, physiopathology Th1-Th2 Balance |
| Grant Support | |
ID/Acronym/Agency:
|
R01 AI072502-01A1/AI/NIAID NIH HHS; R01 AI072502-02/AI/NIAID NIH HHS; R01 AI072502-04/AI/NIAID NIH HHS; R01 AI072502-05/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/CCL26 protein, human; 0/Chemokines, CC; 0/Interleukin-13; 67256-21-7/Hepatocyte Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nucleic acid extraction efficiency and bacterial recovery from maxillary sinus mucosal samples obtai...
Next Document: Clinical and immunologic effects of sublingual immunotherapy on patients with allergic rhinitis to h...